da Rocha Raphael Francisco Dutra Barbosa, LaRocque-de-Freitas Isabel Ferreira, Arcanjo Angelica Fernandes, Logullo Jorgete, Nunes Marise Pinheiro, Freire-de-Lima Celio Geraldo, Decote-Ricardo Debora
Instituto de Veterinária, Universidade Federal Rural do Rio de Janeiro, Rio de Janeiro, Brazil.
Instituto de Biofísica Carlos Chagas Filho, Universidade Federal do Rio de Janeiro, Rio de Janeiro, Brazil.
Front Microbiol. 2019 Jul 9;10:1598. doi: 10.3389/fmicb.2019.01598. eCollection 2019.
B-1 cells can directly and indirectly influence the immune response. These cells are known to be excellent producers of natural antibodies and can secrete a variety of immunomodulatory molecules. They are also able to differentiate into B-1 cell-derived phagocytes (B-1CDP). B-1 cells can modulate macrophages to become less effective, and B-1CDP cells are more susceptible in infection models. In this work, we investigated the microbicidal ability of these cells in infection . The results show that macrophages from BALB/c mice are more susceptible to infection than macrophages from XID mice. The resistance observed in macrophages from XID mice was abolished in the presence of B-1 cells, and this event seems to be associated with IL-10 production by B-1 cells, which may have contributed to the decrease of NO production. Additionally, B-1CDP cells were more permissive to intracellular infection than peritoneal macrophages. These findings strongly suggest that B-1 cells and B-1CDP cells have a potential role in the persistence of the parasite in host cells.
B-1细胞可直接和间接影响免疫反应。已知这些细胞是天然抗体的优秀产生者，并且能够分泌多种免疫调节分子。它们还能够分化为B-1细胞衍生的吞噬细胞（B-1CDP）。B-1细胞可调节巨噬细胞，使其效力降低，而在感染模型中，B-1CDP细胞更易受到感染。在这项研究中，我们调查了这些细胞在感染中的杀菌能力。结果显示，BALB/c小鼠的巨噬细胞比XID小鼠的巨噬细胞更易受到感染。在存在B-1细胞的情况下，XID小鼠巨噬细胞所观察到的抗性消失了，这一现象似乎与B-1细胞产生的IL-10有关，IL-10可能导致了一氧化氮产生的减少。此外，B-1CDP细胞比腹膜巨噬细胞更易受到细胞内感染。这些发现有力地表明，B-1细胞和B-1CDP细胞在寄生虫在宿主细胞中的持续存在方面具有潜在作用。
