Greenberg M M
Department of Chemistry, Johns Hopkins University, 3400 North Charles St, Baltimore, MD 21218, USA.
Biochem Soc Trans. 2004 Feb;32(Pt 1):46-50. doi: 10.1042/bst0320046.
Biochemical, biophysical and biological studies of oligonucleotides containing lesions at defined sites provide a molecular basis for the effects of DNA lesions. dG (deoxyguanosine) is the most easily oxidized of the four native nucleotides. The chemical reactivity of dG correlates with compilations of mutations, which reveal that a significant fraction of transitions or transversions involve dG. OxodG (7,8-dihydro-8-hydroxy-2'-deoxyguanosine) is widely recognized as an important lesion derived from the oxidation of dG, and significant effort has been expended in studies of its effects on DNA structure and function. Recently, the properties of other lesions derived from dG and/or the oxidation of OxodG have been uncovered. Studies on these lesions reveal that they too are biologically significant.
对在特定位点含有损伤的寡核苷酸进行的生化、生物物理和生物学研究为DNA损伤的影响提供了分子基础。脱氧鸟苷（dG）是四种天然核苷酸中最易被氧化的。dG的化学反应性与突变汇编相关，这些汇编表明相当一部分转换或颠换涉及dG。氧化鸟苷（7,8-二氢-8-羟基-2'-脱氧鸟苷，OxodG）被广泛认为是由dG氧化产生的一种重要损伤，并且在研究其对DNA结构和功能的影响方面已经投入了大量精力。最近，其他源自dG和/或OxodG氧化的损伤的特性也被发现。对这些损伤的研究表明它们在生物学上也具有重要意义。
