Guichardant M, Chantegrel B, Deshayes C, Doutheau A, Moliere P, Lagarde M
UMR 585 INSERM/INSA-Lyon, Bldg Louis Pasteur, 20 Avenue A. Einstein, 69621 Villeurbanne, France.
Biochem Soc Trans. 2004 Feb;32(Pt 1):139-40. doi: 10.1042/bst0320139.
Several markers of lipid peroxidation are available with different degrees of specificity, from malondialdehyde as a global marker, to F(2)-isoprostane, which is specifically produced from arachidonic acid. Among these, 4-hydroxynonenal is recognized as a breakdown product of fatty acid hydroperoxides, such as 15-hydroperoxy-eicosatetraenoic acid and 13-hydroperoxy-octade cadienoic acid from the n -6 fatty acids. Furthermore, 4-hydroxyhexenal (4-HHE) derives from n -3 fatty acid hydroperoxides. We have recently described the occurrence of 4-hydroxydodecadienal (4-HDDE) from the 12-lipoxygenase product of arachidonic acid 12-hydroperoxy-eicosatetraenoic acid. These three hydroxy-alkenals may be measured in human plasma by GC-MS, but they may partly be generated in the course of sampling, and the relative volatility of 4-HHE makes its measurement quite unreliable. We have successfully characterized and measured the stable oxidized carboxylic acid products from the hydroxy-alkenals 4-HNA, 4-HHA and 4-HDDA in urine. The ratio between 4-HHA and 4-HNA found in the same urinary sample might provide useful information on the location of lipid peroxidation, accounting for the high enrichment of the cerebrovascular system with docosahexaenoic acid, the main n -3 fatty acid in humans.
有几种脂质过氧化标志物，其特异性程度各不相同，从作为整体标志物的丙二醛到由花生四烯酸特异性产生的F(2)-异前列腺素。其中，4-羟基壬烯醛被认为是脂肪酸氢过氧化物的分解产物，例如来自n-6脂肪酸的15-氢过氧-二十碳四烯酸和13-氢过氧-十八碳二烯酸。此外，4-羟基己烯醛（4-HHE）源自n-3脂肪酸氢过氧化物。我们最近描述了花生四烯酸12-氢过氧-二十碳四烯酸的12-脂氧合酶产物中4-羟基十二碳二烯醛（4-HDDE）的出现。这三种羟基烯醛可以通过气相色谱-质谱联用仪在人体血浆中进行测量，但它们可能在采样过程中部分产生，并且4-HHE的相对挥发性使其测量相当不可靠。我们已经成功地对尿液中羟基烯醛4-HNA、4-HHA和4-HDDA的稳定氧化羧酸产物进行了表征和测量。在同一尿液样本中发现的4-HHA与4-HNA的比值可能提供有关脂质过氧化位置的有用信息，这是由于脑血管系统中富含二十二碳六烯酸，这是人类主要的n-3脂肪酸。
