Soustre Jérôme, Rodier Marie-Hélène, Imbert-Bouyer Sabrina, Daniault Gyslaine, Imbert Christine
Laboratoire de Parasitologie et Mycologie Médicales, Centre Hospitalier Universitaire La Milétrie, BP 577, 86021 Poitiers Cedex, France.
J Antimicrob Chemother. 2004 Mar;53(3):522-5. doi: 10.1093/jac/dkh099. Epub 2004 Jan 28.
Some manifestations of candidiasis are associated with the formation of biofilms on inert or biological surfaces and the intrinsic resistance of Candida albicans biofilms to the most commonly used antifungal agents has been demonstrated. In this study, we report on the influence of the growth of C. albicans in medium containing a sub-inhibitory concentration (MIC/2) of caspofungin, on subsequent fungal adherence to plastic coated with extracellular matrix (ECM) proteins.
Eleven strains of C. albicans were studied: six strains were susceptible to fluconazole in vitro and five strains were resistant to this antifungal agent.
Caspofungin induced a decrease in the adherence of all the tested strains that were susceptible to fluconazole but induced a decrease in the adherence of only 60% of the fluconazole-resistant strains.
This study demonstrated the anti-adherent activity of caspofungin but indicated a reduced effect in the case of in vitro fluconazole resistance. These results indicated a possible relationship between the efficiency of caspofungin to inhibit the first step of the development of C. albicans biofilm and the resistance of C. albicans to fluconazole in vitro.
念珠菌病的一些表现与在惰性或生物表面形成生物膜有关，并且白色念珠菌生物膜对最常用抗真菌药物的固有抗性已得到证实。在本研究中，我们报告了白色念珠菌在含有亚抑菌浓度（MIC/2）卡泊芬净的培养基中生长对随后真菌黏附于包被有细胞外基质（ECM）蛋白的塑料的影响。
研究了11株白色念珠菌：6株在体外对氟康唑敏感，5株对该抗真菌药物耐药。
卡泊芬净使所有对氟康唑敏感的受试菌株的黏附减少，但仅使60%的氟康唑耐药菌株的黏附减少。
本研究证明了卡泊芬净的抗黏附活性，但表明在体外氟康唑耐药的情况下效果降低。这些结果表明卡泊芬净抑制白色念珠菌生物膜形成第一步的效率与白色念珠菌体外对氟康唑的耐药性之间可能存在关联。
