Murray Martha Meaney, Zurakowski David, Vrahas Mark S
Department of Orthopadic Surgery, Brigham and Women's Hospital, Harvard Medical School, Boston, MA 02115, USA.
J Trauma. 2004 Jan;56(1):128-31. doi: 10.1097/01.TA.0000051934.96670.37.
In this study, we wished to determine whether chondrocyte death occurs after intra-articular fracture and might thus contribute to the development of posttraumatic arthritis.
Articular fracture fragments were obtained from 30 patients and the terminal deoxynucleotide transferase-mediated dUTP nick-end labeling assay was used to identify dead or dying cells.
Dead or dying cells were identified in all fracture fragments, with an average rate of cell death of 35%. Rates of cell death greater than 90% were seen in 4 of the 30 specimens studied.
Although rates of apoptosis in osteoarthritis have been reported to average 15%, the rate in this fracture population was more than twice this value (35%). The high rate of cell death noted here may help explain the occurrence of posttraumatic arthritis even in anatomically fixed intra-articular fractures.
在本研究中，我们希望确定关节内骨折后是否会发生软骨细胞死亡，以及这是否可能导致创伤后关节炎的发展。
从30例患者获取关节骨折碎片，并采用末端脱氧核苷酸转移酶介导的dUTP缺口末端标记法鉴定死亡或濒死细胞。
在所有骨折碎片中均发现了死亡或濒死细胞，平均细胞死亡率为35%。在研究的30个标本中，有4个标本的细胞死亡率超过90%。
尽管据报道骨关节炎中的细胞凋亡率平均为15%，但该骨折人群中的细胞凋亡率是此值的两倍多（35%）。此处观察到的高细胞死亡率可能有助于解释即使在解剖复位的关节内骨折中创伤后关节炎的发生。
