Thaller M C, Berlutti F, Riccio M L, Rossolini G M
Istituto di Microbiologia, Università La Sapienza, Rome, Italy.
Mol Cell Probes. 1992 Oct;6(5):417-22. doi: 10.1016/0890-8508(92)90036-w.
A DNA probe is described that can be used for identification of Providencia stuartii by means of filter hybridization assays. The probe, which is a fragment of the P. stuartii phoN gene coding for an acid phosphatase, appeared to be able to recognize only P. stuartii strains in slot-blot hybridization experiments performed with total DNA extracted from 545 strains of 64 different Gram-negative bacterial species, including all the major representatives of the family Enterobacteriaceae. Owing to the problems that may be often encountered for correct identification of P. stuartii at the species level when using commercial identification systems, this probe may result useful for fast and reliable identification of P. stuartii strains for taxonomical, epidemiological and diagnostic studies.
描述了一种DNA探针，可用于通过滤膜杂交试验鉴定斯氏普罗威登斯菌。该探针是编码酸性磷酸酶的斯氏普罗威登斯菌phoN基因的一个片段，在对从64种不同革兰氏阴性细菌的545株菌株（包括肠杆菌科所有主要代表菌株）中提取的总DNA进行的狭缝印迹杂交实验中，似乎只能识别斯氏普罗威登斯菌菌株。由于使用商业鉴定系统在物种水平正确鉴定斯氏普罗威登斯菌时经常会遇到问题，该探针可能有助于在分类学、流行病学和诊断研究中快速可靠地鉴定斯氏普罗威登斯菌菌株。
