Thaller M C, Berlutti F, Pantanella F, Pompei R, Satta G
Istituto di Microbiologia, Università La Sapienza, Rome, Italy.
J Clin Microbiol. 1992 Aug;30(8):2054-7. doi: 10.1128/jcm.30.8.2054-2057.1992.
This work describes a modified MacConkey medium (MCP medium) enabling the simple identification of Providencia stuartii, an emerging nosocomial pathogen. A total of 813 strains, belonging to the families Enterobacteriaceae and Pseudomonadaceae, were tested on MCP medium; all P. stuartii strains were phosphatase positive, as were 97.5% of Morganella morganii strains, in contrast with all other tested organisms. A simple discriminating test, such as the ornithine or citrate test, allowed identification of strains of these species. We have also compared the reliabilities of P. stuartii identification by commercial kits (API 20E system) by using a standard MacConkey or MCP medium. Sixteen and three-tenths percent of P. stuartii strains were misidentified by using the former procedure, while with the latter all strains were correctly identified. Finally, the MCP medium was used over a 6-month period in our routine clinical laboratory. Of a total of 1,278 seeded urine samples from elderly patients, we isolated 103 P. stuartii strains which were all correctly identified by coupling MCP medium and the API 20E system. Seventeen and one-half percent of these strains were misidentified when the API 20E system was used in combination with standard MacConkey medium.
本研究描述了一种改良的麦康凯培养基（MCP培养基），可用于简单鉴定新兴医院病原体斯氏普罗威登斯菌。共对813株属于肠杆菌科和假单胞菌科的菌株在MCP培养基上进行了测试；所有斯氏普罗威登斯菌菌株磷酸酶呈阳性，摩根氏摩根菌菌株的这一比例为97.5%，而其他所有测试菌株均为阴性。一项简单的鉴别试验，如鸟氨酸或柠檬酸盐试验，可用于鉴定这些菌种的菌株。我们还比较了使用标准麦康凯培养基或MCP培养基通过商业试剂盒（API 20E系统）鉴定斯氏普罗威登斯菌的可靠性。使用前一种方法时，16.3%的斯氏普罗威登斯菌菌株被误鉴定，而使用后一种方法时，所有菌株均被正确鉴定。最后，MCP培养基在我们的常规临床实验室中使用了6个月。在总共1278份老年患者的接种尿样中，我们分离出103株斯氏普罗威登斯菌菌株，通过将MCP培养基与API 20E系统结合使用，所有菌株均被正确鉴定。当API 20E系统与标准麦康凯培养基联合使用时，这些菌株中有17.5%被误鉴定。
