Myers W D, Ludden P A, Nayigihugu V, Hess B W
Department of Animal Science, University of Wyoming, Laramie 82071-3684, USA.
J Anim Sci. 2004 Jan;82(1):179-83. doi: 10.2527/2004.821179x.
A procedure was developed for the rapid analysis of titanium dioxide (TiO2) concentrations in feed and fecal samples. Samples were digested in concentrated H2SO4 for 2 h, followed by addition of 30% H2O2, and absorbance was measured at 410 nm. Standards were prepared by spiking blanks with increasing amounts of TiO2, resulting in a linear standard curve. Complete analysis using this procedure can typically be accomplished within 4.5 h. This procedure was compared to a previously published dry-ash procedure for the analysis of TiO2 in bovine fecal samples. Three sources of OM devoid of TiO2 (a forage sample, a bovine fecal sample without Cr2O3, and a bovine fecal sample containing Cr2O3) were spiked with graded amounts (0, 2, 4, 6, 8, or 10 mg) of TiO2. With our procedure, TiO2 recoveries averaged 96.7, 97.5, and 98.5%, for the three OM sources, respectively, vs. 74.3, 83.8, and 53.1% for the same samples analyzed using the dry-ash method. These results suggest that our procedure is a rapid and accurate alternative to dry-ash procedures for the determination of TiO2.
开发了一种用于快速分析饲料和粪便样品中二氧化钛（TiO₂）浓度的方法。样品在浓硫酸中消化2小时，然后加入30%的过氧化氢，并在410nm处测量吸光度。通过向空白样品中加入逐渐增加量的TiO₂来制备标准品，从而得到一条线性标准曲线。使用该方法进行完整分析通常可在4.5小时内完成。将该方法与之前发表的用于分析牛粪便样品中TiO₂的干灰化方法进行了比较。向三种不含TiO₂的有机物质来源（一种饲料样品、一种不含Cr₂O₃的牛粪便样品和一种含有Cr₂O₃的牛粪便样品）中加入不同量（0、2、4、6、8或10mg）的TiO₂。使用我们的方法，三种有机物质来源的TiO₂回收率分别平均为96.7%、97.5%和98.5%，而使用干灰化法分析相同样品时的回收率分别为74.3%、83.8%和53.1%。这些结果表明，我们的方法是一种用于测定TiO₂的快速且准确的替代干灰化方法。
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