Xie Xiao-zhi, Wang Zong-min, Zhang Hai-yan, Wang Lan, Gao Bao-hui, Li Xue-mei, Hu Wei-guo
Department of Gastroenterology, Yuying Children's Hospital, Wenzhou Medical College, Wenzhou 325027, China.
Zhonghua Er Ke Za Zhi. 2006 Oct;44(10):774-7.
Since application of pediatric gastroscopy in the mid-nineteen nineties, there has been a trend that the prevalence rates of pediatric gastritis and duodenal ulcer (DU) are increasing. The diagnosed rate of pediatric gastritis has accounted for 85% - 95% of the total number of children who received gastroscopy, and the rate of DU accounted for 8% - 22%. Such a high rates of the diseases may influence the development of the children severely. However, the etiology and pathogenesis of pediatric chronic gastritis and DU have not been completely elucidated. The disordered gastrointestinal hormones play a crucial role in the pediatric chronic gastritis and DU. This study focused on the expression of gastrin (GAS), somatostatin (SS) in the mucosa of gastric antrum and PCNA and Fas-L in the sinus ventriculi and their possible roles in the pathogenesis of pediatric chronic gastritis and DU.
The sinus ventriculi mucosal samples of 83 cases were collected via gastroscopic biopsy from the hospital during the recent two years and the cases were divided into five groups: group A, chronic superficial gastritis, Helicobacter pylori (Hp)(+); group B, chronic superficial gastritis, Hp(-); group C, DU, Hp(+); Group D, DU, Hp(-); Group E, normal sinus ventriculi mucosa, Hp(-). Immunohistochemical staining (En Vision) was carried out for GAS, SS, PCNA and Fas-L, and positive cells of each slide were counted (x 400). Statistically significant differences among groups for continuous data were assessed with the software SPSS10.0.
The expressions of GAS and SS in the groups A through E had no significant difference. The expression of PCNA in group A was significantly higher than that in group B (P < 0.05), and no significant differences were found among the other groups. There were no significant differences in expressions of Fas-L among the five groups.
There seems to be an increasing tendency in the expressions of GAS and SS in children with chronic gastritis and duodenal ulcer. Hp infection promotes the multiplication of the sinus ventriculi mucosal epithelium cells in the pediatric chronic gastritis.
自20世纪90年代中期小儿胃镜应用以来，小儿胃炎和十二指肠溃疡（DU）的患病率有上升趋势。小儿胃炎的诊断率占接受胃镜检查儿童总数的85% - 95%，DU的诊断率占8% - 22%。如此高的发病率可能严重影响儿童的发育。然而，小儿慢性胃炎和DU的病因及发病机制尚未完全阐明。胃肠激素紊乱在小儿慢性胃炎和DU中起关键作用。本研究聚焦于胃窦黏膜中胃泌素（GAS）、生长抑素（SS）以及胃体中增殖细胞核抗原（PCNA）和Fas配体（Fas-L）的表达及其在小儿慢性胃炎和DU发病机制中的可能作用。
收集近两年在本院经胃镜活检获取的83例胃体黏膜样本，将病例分为五组：A组，慢性浅表性胃炎，幽门螺杆菌（Hp）（+）；B组，慢性浅表性胃炎，Hp（-）；C组，DU，Hp（+）；D组，DU，Hp（-）；E组，正常胃体黏膜，Hp（-）。对GAS、SS、PCNA和Fas-L进行免疫组织化学染色（En Vision法），每张切片在400倍视野下计数阳性细胞。使用SPSS10.0软件评估连续数据在各组间的统计学显著差异。
A组至E组GAS和SS的表达无显著差异。A组PCNA的表达显著高于B组（P < 0.05），其他组间无显著差异。五组Fas-L的表达无显著差异。
慢性胃炎和十二指肠溃疡患儿中GAS和SS的表达似乎有上升趋势。Hp感染促进小儿慢性胃炎中胃体黏膜上皮细胞的增殖。
