Nostro Antonia, Blanco Anna R, Cannatelli Maria A, Enea Vincenzo, Flamini Guido, Morelli Ivano, Sudano Roccaro Andrea, Alonzo Vittorio
Dipartimento Farmaco-Biologico, Sezione Microbiologia, Facoltà di Farmacia, Università di Messina, Villaggio Annunziata, 98168 Messina, Italy.
FEMS Microbiol Lett. 2004 Jan 30;230(2):191-5. doi: 10.1016/S0378-1097(03)00890-5.
The aim of this study was to evaluate the susceptibility of methicillin-susceptible and methicillin-resistant staphylococci (MSS, MRS) to oregano essential oil, carvacrol and thymol. The commercial aerial parts of Origanum vulgare L. were hydrodistilled and the essential oil analysed by gas- chromatography/electron impact mass spectrometry. The inhibition efficacy of this essence and its major components was assayed against 26 MSS and 21 MRS, using an agar dilution method. The methicillin resistance was thoroughly typed by Epsilometer test (E-test), polymerase chain reaction for mecA gene detection and PBP2' latex agglutination test. The results clearly demonstrated that the comparison between the susceptibility of MSS and MRS to oregano oil, carvacrol and thymol showed no significant differences (Fisher's exact test, P > 0.05). The best minimum inhibitory concentration values were reported for carvacrol (0.015-0.03%, v/v) followed by thymol (0.03-0.06%, v/v) and oregano oil (0.06-0.125%, v/v).
本研究旨在评估甲氧西林敏感和耐甲氧西林葡萄球菌（MSS、MRS）对牛至精油、香芹酚和百里香酚的敏感性。对牛至商业地上部分进行水蒸馏，并采用气相色谱/电子轰击质谱法分析精油。使用琼脂稀释法测定该精油及其主要成分对26株MSS和21株MRS的抑制效果。通过Epsilometer试验（E试验）、用于检测mecA基因的聚合酶链反应和PBP2'乳胶凝集试验对甲氧西林耐药性进行全面分型。结果清楚地表明，MSS和MRS对牛至油、香芹酚和百里香酚敏感性的比较无显著差异（Fisher精确检验，P>0.05）。报道的最佳最低抑菌浓度值为香芹酚（0.015-0.03%，v/v），其次是百里香酚（0.03-0.06%，v/v）和牛至油（0.06-0.125%，v/v）。
