Mosleh Y Y, Paris-Palacios S, Arnoult F, Couderchet M, Biagianti-Risbourg S, Vernet G
Laboratoire d'Eco-Toxicologie, Unité de Recherche sur la Vigne et le Vin de Champagne-UPRES-EA 2069, Faculté des Sciences, Université de Reims Champagne-Ardenne, B.P. 1039, 51687 Reims cedex 02, France.
Environ Toxicol. 2004 Feb;19(1):88-93. doi: 10.1002/tox.10153.
Metallothioneins (MTs) are low-molecular-weight proteins mainly involved in metal ion detoxification. Recently it has been demonstrated that MTs participate in several cellular functions such as regulation of growth and antioxidative defenses. Moreover, pesticides can induce their synthesis. The aim of the current work was to determine the effects of isoproturon, either pure or formulated as Matin (suspension containing an isoproturon concentration of 500 g. L(-1)), on the metallothionein and total protein contents of the aquatic worm Tubifex tubifex. MT levels in exposed worms increased significantly after 7 and 15 days of exposure to a concentration of the herbicide of 50 mg. L(-1). Isoproturon reduced the metal (Cu, Zn, and Cd) content of metallothioneins, and it also increased the total protein content of the worms. These results suggest that MT induction may not be considered a specific biomarker of metal exposure but that it can be used as a nonspecific biomarker of the effect of isoproturon effect in aquatic worms.
金属硫蛋白（MTs）是主要参与金属离子解毒的低分子量蛋白质。最近有研究表明，MTs参与多种细胞功能，如生长调节和抗氧化防御。此外，农药可诱导其合成。本研究的目的是确定异丙隆（无论是纯品还是配制成马丁（一种含500 g·L⁻¹异丙隆浓度的悬浮剂））对水生蠕虫颤蚓金属硫蛋白和总蛋白含量的影响。暴露于50 mg·L⁻¹除草剂浓度7天和15天后，暴露蠕虫体内的MT水平显著增加。异丙隆降低了金属硫蛋白中的金属（铜、锌和镉）含量，同时也增加了蠕虫的总蛋白含量。这些结果表明，MT诱导可能不能被视为金属暴露的特异性生物标志物，但它可作为异丙隆对水生蠕虫影响的非特异性生物标志物。
