Cánovas Francisco M, Dumas-Gaudot Eliane, Recorbet Ghislaine, Jorrin Jesus, Mock Hans-Peter, Rossignol Michel
Departamento de Biología Molecular y Bioquímica, Instituto Andaluz de Biotecnología, Unidad Asociada UMA-CSIC, Universidad de Málaga, Málaga, Spain.
Proteomics. 2004 Feb;4(2):285-98. doi: 10.1002/pmic.200300602.
Proteome analysis is becoming a powerful tool in the functional characterization of plants. Due to the availability of vast nucleotide sequence information and based on the progress achieved in sensitive and rapid protein identification by mass spectrometry, proteome approaches open up new perspectives to analyze the complex functions of model plants and crop species at different levels. In this review, an overview is given on proteome studies performed to analyze whole plants or specific tissues with particular emphasis on important physiological processes such as germination. The chapter on subcellular proteome analysis of plants focuses on the progress achieved for plastids and mitochondria but also mentions the difficulties associated with membrane-bound proteins of these organelles. Separate chapters are dedicated to the challenging analysis of woody plants and to the use of proteome approaches to investigate the interaction of plants with pathogens or with symbiotic organisms. Limitations of current techniques and recent conceptual and technological perspectives for plant proteomics are briefly discussed in the final chapter.
蛋白质组分析正成为植物功能特性研究中的一项强大工具。由于海量核苷酸序列信息的可得性，且基于质谱技术在灵敏快速蛋白质鉴定方面取得的进展，蛋白质组学方法为在不同层面分析模式植物和作物物种的复杂功能开辟了新的视角。在本综述中，概述了为分析整株植物或特定组织而开展的蛋白质组学研究，特别强调了诸如萌发等重要生理过程。关于植物亚细胞蛋白质组分析的章节聚焦于质体和线粒体方面取得的进展，但也提及了与这些细胞器膜结合蛋白相关的困难。单独的章节专门论述木本植物的挑战性分析以及利用蛋白质组学方法研究植物与病原体或共生生物的相互作用。最后一章简要讨论了当前技术的局限性以及植物蛋白质组学近期的概念和技术展望。
