Laboratory of Pharmacology and Inflammation, FACFAN/Federal University of Mato Grosso do Sul, Campo Grande, Mato Grosso do Sul, Brazil.
Multicenter Program of Post-Graduation in Biochemistry and Molecular Biology, INBIO/Federal University of Mato Grosso do Sul, Campo Grande, Mato Grosso do Sul, Brazil.
Inflammation. 2019 Dec;42(6):2011-2019. doi: 10.1007/s10753-019-01062-6.
Stings from the wasp Parachartergus fraternus occur throughout Latin America, and edema followed by pain is the main symptom presented by victims. This often limited inflammatory event has not been characterized for this species. In this work, we identified the mechanisms and possible mediators involved in this response. P. fraternus venom (100, 200, and 400 μg/kg) was injected into the hind paws of mice, and edema was evaluated at intervals of 10 min for up to 60 min and at 120, 240, and 1440 min using a digital plethysmometer. The peak of edema was observed at 10 min with a dose of 200 μg/kg. A reduction in edema was observed with indomethacin (58.1%), celecoxib (44.5%), MK886 (30.8%), and dexamethasone (53.2%). Loratadine, cimetidine, and cyproheptadine treatment reduced the edema by 54.2%, 63.9%, and 84.4%, respectively, compared with the control. Captopril and L-NAME inhibited 42.5% and 69.8%, respectively, of the edema. These results showed that the edema induced in mice by P. fraternus venom occurs early and is mediated by arachidonic acid derivatives, vasoactive amines, and nitric oxide. Together, these mediators amplify the inflammatory process, with emphasis on histamine and serotonin in triggering the edematogenic response, being more effective the use of cyproheptadine in the therapeutic approach.
美洲大虎头蜂的蛰伤遍及拉丁美洲，受害者的主要症状是随后出现的肿胀和疼痛。这种常局限的炎症反应尚未对此种蜂的毒液特征进行描述。在这项工作中，我们鉴定了涉及该反应的机制和可能的介质。将美洲大虎头蜂毒液（100、200 和 400μg/kg）注射到小鼠的后爪中，使用数字体积描记器在 10 分钟的间隔内评估肿胀，直到 60 分钟和 120、240 和 1440 分钟。在 200μg/kg 的剂量下观察到肿胀的峰值出现在 10 分钟。用吲哚美辛（58.1%）、塞来昔布（44.5%）、MK886（30.8%）和地塞米松（53.2%）治疗观察到肿胀减少。与对照组相比，氯雷他定、西咪替丁和赛庚啶治疗分别使肿胀减少了 54.2%、63.9%和 84.4%。卡托普利和 L-NAME 分别抑制了 42.5%和 69.8%的肿胀。这些结果表明，美洲大虎头蜂毒液诱导的小鼠肿胀发生较早，由花生四烯酸衍生物、血管活性胺和一氧化氮介导。这些介质共同放大炎症过程，组胺和 5-羟色胺在引发水肿反应中起重要作用，在治疗方法中使用赛庚啶更有效。
