Veveris Maris, Koch Egon, Chatterjee Shyam S
Department of Medicinal Chemistry, Latvian Institute of Organic Synthesis, Riga, Latvia.
Life Sci. 2004 Feb 27;74(15):1945-55. doi: 10.1016/j.lfs.2003.09.050.
In Germany, hydroalcoholic extracts from hawthorn (Crataegus spp.) leaves with flowers are approved drugs for the treatment of mild forms of heart insufficiency. Besides cardiotonic effects these herbal remedies have been shown to possess cardioprotective properties. We now evaluated if treatment of rats with the Crataegus special extract WS 1442 also improves cardiac function and prevents myocardial infarction during prolonged ischemia and reperfusion lasting for 240 and 15 min, respectively. Oral administration of WS 1442 (10 or 100 mg x kg(-1) x day(-1)) for 7 days before ligation of the left coronary artery dose-dependently suppressed the decrease of the pressure rate product. WS 1442 treatment also attenuated the elevation of the ST-segment in the ECG, diminished the incidence of ventricular fibrillations (control: 67%; 10 mg x kg(-1): 64%; 100 mg x kg(-1): 27%) and reduced the mortality rate (control: 47%; 10 mg.kg(-1): 27%; 100 mg x kg(-1): 9%). Furthermore, the area of myocardial infarction within the ischemic zone was significantly smaller in treated rats (10 mg x kg(-1): 64.3 +/- 5.1%; 100 mg x kg(-1): 42.8 +/- 4.1%) when compared with controls (78.4 +/- 2.6%). It is suggested that these pharmacological effects are accounted for by the combined antioxidative, leukocyte elastase inhibiting and endothelial nitric oxide (NO) synthesis enhancing properties of WS 1442.
在德国，山楂（Crataegus spp.）带花叶片的水醇提取物是治疗轻度心力衰竭的获批药物。除了强心作用外，这些草药制剂还具有心脏保护特性。我们现在评估用山楂特殊提取物WS 1442治疗大鼠是否也能改善心脏功能，并在分别持续240分钟和15分钟的长时间缺血和再灌注过程中预防心肌梗死。在结扎左冠状动脉前7天，口服WS 1442（10或100 mg·kg⁻¹·d⁻¹）剂量依赖性地抑制压力速率乘积的下降。WS 1442治疗还减轻了心电图中ST段的抬高，降低了心室颤动的发生率（对照组：67%；10 mg·kg⁻¹：64%；100 mg·kg⁻¹：27%），并降低了死亡率（对照组：47%；10 mg·kg⁻¹：27%；100 mg·kg⁻¹：9%）。此外，与对照组（78.4±2.6%）相比，治疗组大鼠缺血区内的心肌梗死面积明显较小（10 mg·kg⁻¹：64.3±5.1%；100 mg·kg⁻¹：42.8±4.1%）。提示这些药理作用是由WS 1442的抗氧化、抑制白细胞弹性蛋白酶和增强内皮型一氧化氮（NO）合成的联合特性所致。
