了哥王中双黄酮类化合物的体外抗疟活性。

In vitro antimalarial activity of biflavonoids from Wikstroemia indica.

作者信息

Nunome Shinyu, Ishiyama Aki, Kobayashi Miyuki, Otoguro Kazuhiko, Kiyohara Hiroaki, Yamada Haruki, Omura Satoshi

机构信息

The Kitasato Institute for Life Sciences, Kitasato University, Tokyo, Japan.

出版信息

Planta Med. 2004 Jan;70(1):76-8. doi: 10.1055/s-2004-815462.

DOI:10.1055/s-2004-815462

PMID:14765300

Abstract

In our investigation of in vitro antimalarial screening of medicinal herbal extracts, the n-BuOH extract from the root of Wikstroemia indica showed a potent inhibitory effect. Fractionation of the active extract led to the isolation of two biflavonoids, sikokianin B ( 1) and sikokianin C ( 2) with IC (50) values 0.54 microg/mL and 0.56 microg/mL, respectively, against the chloroquine-resistant strain of Plasmodium falciparum. This is the first report of the biological activity of 1 and 2. As the structure of l has remained unsettled, we confirmed the conformation by (1)H- and (13)C-NMR.

摘要

在我们对药草提取物的体外抗疟筛选研究中，了哥王根的正丁醇提取物显示出强效抑制作用。对活性提取物进行分离得到了两种双黄酮，即 sikokianin B（1）和 sikokianin C（2），它们对恶性疟原虫氯喹抗性株的半数抑制浓度（IC50）值分别为 0.54 μg/mL 和 0.56 μg/mL。这是关于 1 和 2 生物活性的首次报道。由于 1 的结构尚未确定，我们通过氢核磁共振（1H-NMR）和碳核磁共振（13C-NMR）确定了其构象。