Nunome Shinyu, Ishiyama Aki, Kobayashi Miyuki, Otoguro Kazuhiko, Kiyohara Hiroaki, Yamada Haruki, Omura Satoshi
The Kitasato Institute for Life Sciences, Kitasato University, Tokyo, Japan.
Planta Med. 2004 Jan;70(1):76-8. doi: 10.1055/s-2004-815462.
In our investigation of in vitro antimalarial screening of medicinal herbal extracts, the n-BuOH extract from the root of Wikstroemia indica showed a potent inhibitory effect. Fractionation of the active extract led to the isolation of two biflavonoids, sikokianin B ( 1) and sikokianin C ( 2) with IC (50) values 0.54 microg/mL and 0.56 microg/mL, respectively, against the chloroquine-resistant strain of Plasmodium falciparum. This is the first report of the biological activity of 1 and 2. As the structure of l has remained unsettled, we confirmed the conformation by (1)H- and (13)C-NMR.
在我们对药草提取物的体外抗疟筛选研究中，了哥王根的正丁醇提取物显示出强效抑制作用。对活性提取物进行分离得到了两种双黄酮，即 sikokianin B（1）和 sikokianin C（2），它们对恶性疟原虫氯喹抗性株的半数抑制浓度（IC50）值分别为 0.54 μg/mL 和 0.56 μg/mL。这是关于 1 和 2 生物活性的首次报道。由于 1 的结构尚未确定，我们通过氢核磁共振（1H-NMR）和碳核磁共振（13C-NMR）确定了其构象。
