Bano Nasreen, Ruffin Shomari, Ransom Briana, Hollibaugh James T
Department of Marine Sciences, University of Georgia, Athens, Georgia 30602-3636, USA.
Appl Environ Microbiol. 2004 Feb;70(2):781-9. doi: 10.1128/AEM.70.2.781-789.2004.
Archaea assemblages from the Arctic Ocean and Antarctic waters were compared by PCR-denaturing gradient gel electrophoresis (DGGE) analysis of 16S rRNA genes amplified using the Archaea-specific primers 344f and 517r. Inspection of the DGGE fingerprints of 33 samples from the Arctic Ocean (from SCICEX submarine cruises in 1995, 1996, and 1997) and 7 Antarctic samples from Gerlache Strait and Dallman Bay revealed that the richness of Archaea assemblages was greater in samples from deep water than in those from the upper water column in both polar oceans. DGGE banding patterns suggested that most of the Archaea ribotypes were common to both the Arctic Ocean and the Antarctic Ocean. However, some of the Euryarchaeota ribotypes were unique to each system. Cluster analysis of DGGE fingerprints revealed no seasonal variation but supported depth-related differences in the composition of the Arctic Ocean Archaea assemblage. The phylogenetic composition of the Archaea assemblage was determined by cloning and then sequencing amplicons obtained from the Archaea-specific primers 21f and 958r. Sequences of 198 clones from nine samples covering three seasons and all depths grouped with marine group I Crenarchaeota (111 clones), marine group II Euryarchaeota (86 clones), and group IV Euryarchaeota (1 clone). A sequence obtained only from a DGGE band was similar to those of the marine group III Euryarchaeota:
通过聚合酶链反应-变性梯度凝胶电泳（DGGE）分析，利用古菌特异性引物344f和517r扩增的16S rRNA基因，对北冰洋和南极海域的古菌群落进行了比较。对北冰洋的33个样本（来自1995年、1996年和1997年的SCICEX潜艇巡航）以及来自杰拉许海峡和达尔曼湾的7个南极样本的DGGE指纹图谱进行检查后发现，在这两个极地海洋中，来自深水的样本中古菌群落的丰富度高于来自上层水柱的样本。DGGE条带模式表明，大多数古菌核糖型在北冰洋和南极海洋中都很常见。然而，一些广古菌门核糖型在每个系统中都是独特的。对DGGE指纹图谱的聚类分析显示没有季节性变化，但支持了北冰洋古菌群落组成中与深度相关的差异。通过克隆并对从古菌特异性引物21f和958r获得的扩增子进行测序，确定了古菌群落的系统发育组成。来自九个样本（涵盖三个季节和所有深度）的198个克隆的序列与海洋I群泉古菌（111个克隆）、海洋II群广古菌（86个克隆）和IV群广古菌（1个克隆）聚类。仅从一个DGGE条带获得的一个序列与海洋III群广古菌的序列相似：
