White Steven M, Constantin Phillip E, Claycomb William C
Dept. of Biochemistry and Molecular Biology, Louisiana State Univ. Health Sciences Center, 1901 Perdido St., New Orleans, LA 70112, USA.
Am J Physiol Heart Circ Physiol. 2004 Mar;286(3):H823-9. doi: 10.1152/ajpheart.00986.2003.
HL-1 cells are currently the only cardiomyocyte cell line available that continuously divides and spontaneously contracts while maintaining a differentiated cardiac phenotype. Extensive characterization using microscopic, genetic, immunohistochemical, electrophysiological, and pharmacological techniques has demonstrated how similar HL-1 cells are to primary cardiomyocytes. In the few years that HL-1 cells have been available, they have been used in a variety of model systems designed to answer important questions regarding cardiac biology at the cellular and molecular levels. Whereas HL-1 cells have been used to study normal cardiomyocyte function with regard to signaling, electrical, metabolic, and transcriptional regulation, they have also been used to address pathological conditions such as hypoxia, hyperglycemia-hyperinsulinemia, apoptosis, and ischemia-reperfusion. The availability of an immortalized, contractile cardiac cell line has provided investigators with a tool for probing the intricacies of cardiomyocyte function. In this review, we describe the culture and characterization of HL-1 cardiomyocytes as well as various model systems that have been developed using these cells to gain a better understanding of cardiac biology at the cellular and molecular levels.
HL-1细胞是目前唯一可用的心肌细胞系，它能持续分裂并自发收缩，同时保持分化的心脏表型。使用显微镜、遗传学、免疫组织化学、电生理学和药理学技术进行的广泛表征已证明HL-1细胞与原代心肌细胞有多相似。在HL-1细胞可用的短短几年里，它们已被用于各种模型系统，旨在回答有关细胞和分子水平心脏生物学的重要问题。虽然HL-1细胞已被用于研究正常心肌细胞在信号传导、电、代谢和转录调控方面的功能，但它们也被用于研究诸如缺氧、高血糖-高胰岛素血症、细胞凋亡和缺血-再灌注等病理状况。一种永生化的、可收缩的心脏细胞系的出现为研究人员提供了一个探究心肌细胞功能复杂性的工具。在这篇综述中，我们描述了HL-1心肌细胞的培养和表征，以及利用这些细胞开发的各种模型系统，以便在细胞和分子水平上更好地理解心脏生物学。
