四君子汤中的果胶多糖可促进 SW480 细胞的抗氧化防御能力。
A Pectic Polysaccharide from Sijunzi Decoction Promotes the Antioxidant Defenses of SW480 Cells.
机构信息
Laboratory of Experimental Animal Disease Model, College of Veterinary Medicine, Sichuan Agricultural University, Chengdu 611130, China.
Key Laboratory of Animal Disease and Human Health of Sichuan Province, College of Veterinary Medicine, Sichuan Agricultural University, Chengdu 611130, China.
出版信息
Molecules. 2017 Aug 12;22(8):1341. doi: 10.3390/molecules22081341.
Sijunzi Decoction (SJZD) is a formula used for the treatment of spleen deficiency and gastrointestinal diseases in Traditional Chinese Medicine. Polysaccharides are reported to be the main components of SJZD responsible for its bio-functions. However, highly purified and clearly characterized polysaccharides from SJZD are not well described. Here we obtained a purified polysaccharide (SJZDP-II-I) from SJZD using ion exchange chromatography and gel filtration. Structure analysis by FT-IR and NMR identified SJZDP-II-I as a typical pectic polysaccharide with homogalacturonan and rhamnogalacturonan type I regions and arabinogalactan type I and II as side chains. In vitro studies indicated that SJZDP-II-I treatment could significantly enhance the total antioxidant capacity of SW480 cells, resulting from the promoted expressions of antioxidant enzymes and their master regulator PGC-1α, which would be valuable for further research and applications.
四君子汤（SJZD）是一种用于治疗中医脾虚和胃肠道疾病的方剂。据报道，多糖是 SJZD 的主要成分，负责其生物功能。然而，高度纯化和明确表征的 SJZD 多糖尚未得到很好的描述。在这里，我们使用离子交换色谱和凝胶过滤从 SJZD 中获得一种纯化的多糖（SJZDP-II-I）。通过傅里叶变换红外光谱（FT-IR）和核磁共振（NMR）分析，确定 SJZDP-II-I 为一种典型的果胶多糖，具有同型半乳糖醛酸和鼠李半乳糖醛酸 I 型区域以及阿拉伯半乳聚糖 I 和 II 型侧链。体外研究表明，SJZDP-II-I 处理可显著增强 SW480 细胞的总抗氧化能力，这是由于抗氧化酶及其主调控因子 PGC-1α 的表达增强所致，这对于进一步的研究和应用具有重要价值。
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