de Boisséson Claire, Béven Véronique, Bigarré Laurent, Thiéry Richard, Rose Nicolas, Eveno Eric, Madec François, Jestin André
French Food Safety Agency, Viral Genetics and Biosafety Unit, BP 53, F-22 440 Ploufragan, France.
French Food Safety Agency, Fish Infectious Diseases and Parasitology Unit, BP 70, F-29 280 Plouzané, France.
J Gen Virol. 2004 Feb;85(Pt 2):293-304. doi: 10.1099/vir.0.19536-0.
Porcine circovirus type 2 (PCV2) is recognized as a primary cause in post-weaning multisystemic wasting syndrome (PMWS). In this study, both PCV1 and PCV2 types were studied in pigs originating from PMWS-affected (+) and non-affected (-) herds from Brittany. PCV2 was identified by PCR in 100 % of animals from PMWS(+) herds and in 76 % from PMWS(-) herds, while PCV1 was not detected. The complete sequences of 38 PCV2 isolates were determined and 23 new variants were identified, displaying between 94.6 and 99.9 % nucleotide identity with one another. Although highly related to all the PCV2 sequences available in databases, the isolates from France gathered in a distinct subcluster. Compared with the 13 PCV2 from PMWS(+) farms, the 10 PMWS(-) sequences exhibited a slightly higher variability. No viral molecular marker specific to a pathogenic state could be identified, even by including other PCV2 variants isolated from PMWS-suffering animals from other countries. We concluded that the PMWS outbreaks in Brittany are most likely not due to the emergence of a new genotype of circovirus.
猪圆环病毒2型（PCV2）被认为是断奶后多系统消耗综合征（PMWS）的主要病因。在本研究中，对来自布列塔尼受PMWS影响（+）和未受影响（-）猪群的猪进行了PCV1和PCV2两种类型的研究。通过PCR在100%来自PMWS（+）猪群的动物和76%来自PMWS（-）猪群的动物中鉴定出PCV2，而未检测到PCV1。测定了38株PCV2分离株的完整序列，鉴定出23个新变种，它们彼此之间的核苷酸同一性在94.6%至99.9%之间。尽管与数据库中所有可用的PCV2序列高度相关，但来自法国的分离株聚集在一个独特的亚群中。与来自PMWS（+）猪场的13株PCV2相比，10株PMWS（-）序列表现出略高的变异性。即使纳入从其他国家患有PMWS的动物中分离出的其他PCV2变种，也无法鉴定出特定于致病状态的病毒分子标记。我们得出结论，布列塔尼的PMWS疫情很可能不是由于圆环病毒新基因型的出现所致。
