Gundidza G M, Deans S G, Svoboda K P, Mavi S
Department of Pharmacy, Faculty of Medicine, University of Zimbabwe, Harare.
Cent Afr J Med. 1992 Jul;38(7):290-3.
The essential oil from the leaves of Hoslundia opposite was extracted by hydrodistillation. GC/MS analysis of the volatile oil showed that it contains largely the sesquiterpenes and sesquiterpene alcohols. The essential oil was tested against eight different bacterial species and one fungal species. The antibacterial and antifungal properties of the essential oil from Hoslundia opposite were determined by using seeded agar plates with wells into which was placed the oil, and flasks of yeast extract and sucrose broth for mycelial growth of Aspergillus niger. After incubation for 24 hours, the diameter of the inhibition zone was measured for the antibacterial tests and after seven days, the dry weight of the mycelia was measured and a percentage of inhibition calculated using controls where no samples were added. The results obtained showed that the essential oil from this plant has significant activity against Aspergillus niger, Acinetobacter calcoacetica, Brochothrix thermosphacta and Flavobacterium suaveolens. These were most affected by the volatile oil from Hoslundia opposita.
采用水蒸气蒸馏法从对叶肾茶（Hoslundia opposite）叶片中提取挥发油。对挥发油进行气相色谱/质谱（GC/MS）分析表明，其主要成分是倍半萜类和倍半萜醇类。该挥发油针对8种不同细菌和1种真菌进行了测试。采用带孔的接种琼脂平板（向孔中加入挥发油）以及用于黑曲霉菌丝体生长的酵母提取物和蔗糖肉汤烧瓶，测定了对叶肾茶挥发油的抗菌和抗真菌特性。培养24小时后，测量抗菌试验的抑菌圈直径，培养7天后，测量菌丝体干重，并使用未添加样品的对照计算抑制百分比。所得结果表明，该植物的挥发油对黑曲霉、醋酸钙不动杆菌、嗜热栖热放线菌和芳香黄杆菌具有显著活性。这些菌受对叶肾茶挥发油的影响最大。
