评估Syva公司的酶免疫测定法在检测泌尿生殖系统标本中沙眼衣原体的应用。
Evaluation of Syva's enzyme immunoassay for the detection of Chlamydia trachomatis in urogenital specimens.
作者信息
Moncada J, Schachter J, Bolan G, Nathan J, Shafer M A, Clark A, Schwebke J, Stamm W, Mroczkowski T, Seliborska Z
机构信息
Department of Laboratory Medicine, University of California, San Francisco.
出版信息
Diagn Microbiol Infect Dis. 1992 Nov-Dec;15(8):663-8. doi: 10.1016/0732-8893(92)90068-5.
A newly developed microwell enzyme immunosorbent assay (EIA) system by Syva Company (Palo Alto, CA) can detect Chlamydia trachomatis in < 3 hr. It uses a polyclonal antibody to chlamydial lipopolysaccharide and end points are determined with a spectrophotometer. Three clinical trial sites (University of California Medical Center, San Francisco, CA; University of Washington, Seattle, WA; and Louisiana State University Medical Center, New Orleans, LA), compared this EIA with tissue culture (TC) for identifying Chlamydia in urogenital specimens. Overall prevalence by TC was 10.4% (136 of 1306). When tests were compared with TC (using vials or microtiter plates and a fluorescent antibody stain), we found an EIA sensitivity of 93.4% (127 of 136) and a specificity of 98.1% (1148 of 1170). This EIA has a performance profile that is, at the very least, comparable with other nonculture methods for diagnosing genital tract infections with C. trachomatis.
赛瓦公司（加利福尼亚州帕洛阿尔托）新开发的一种微孔酶免疫吸附测定（EIA）系统能够在不到3小时内检测出沙眼衣原体。该系统使用针对衣原体脂多糖的多克隆抗体，并通过分光光度计测定终点。三个临床试验地点（加利福尼亚州旧金山的加利福尼亚大学医学中心；华盛顿州西雅图的华盛顿大学；路易斯安那州新奥尔良的路易斯安那州立大学医学中心）将这种EIA与组织培养（TC）用于泌尿生殖标本中衣原体的鉴定进行了比较。TC检测的总体患病率为10.4%（1306例中的136例）。当将该检测与TC（使用小瓶或微量滴定板以及荧光抗体染色）进行比较时，我们发现EIA的灵敏度为93.4%（136例中的127例），特异性为98.1%（1170例中的1148例）。这种EIA的性能至少与其他用于诊断沙眼衣原体生殖道感染的非培养方法相当。
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