Takemoto M, Shirahama T, Miyauchi T, Matsusako T, Kaneda N, Muramatsu H, Ozawa M, Ohi Y, Muramatsu T
Department of Urology, Faculty of Medicine, Kagoshima University, Japan.
Int J Cancer. 1997 Feb 20;74(1):7-14. doi: 10.1002/(sici)1097-0215(19970220)74:1<7::aid-ijc2>3.0.co;2-6.
A 60-kDa glycoprotein named metanestin was identified by molecular cloning. The glycoprotein had a twice-repeated motif of Pro-Gly-Pro-Gly and carried metastasis-associated carbohydrate epitopes. The antibody to the protein portion of metanestin strongly reacted with lymph-node metastasis of transitional-cell carcinoma of the urinary bladder, but the reactivity to the primary tumor was generally weaker and the normal urothelium was scarcely reactive. Thus both metanestin and the carbohydrate on it showed metastasis-associated expression. In addition, we identified a 100-kDa glycoprotein with a 4-times-repeated motif of Pro-Ala-Pro-Ala. The antibody to the 100-kDa glycoprotein showed reactivity similar to that to metanestin.
通过分子克隆鉴定出一种名为间巢蛋白的60 kDa糖蛋白。该糖蛋白具有Pro-Gly-Pro-Gly的双重重复基序，并携带转移相关的碳水化合物表位。抗间巢蛋白蛋白质部分的抗体与膀胱移行细胞癌的淋巴结转移强烈反应，但对原发性肿瘤的反应性通常较弱，正常尿路上皮几乎无反应。因此，间巢蛋白及其上的碳水化合物均表现出转移相关表达。此外，我们鉴定出一种具有Pro-Ala-Pro-Ala四重重复基序的100 kDa糖蛋白。抗100 kDa糖蛋白的抗体显示出与抗间巢蛋白抗体相似的反应性。
