结肠癌及癌旁正常结肠黏膜中胰腺分泌性胰蛋白酶抑制剂的定量分析。
Quantification of pancreatic secretory trypsin inhibitor in colonic carcinoma and normal adjacent colonic mucosa.
作者信息
Bohe H, Bohe M, Jönsson P, Lindström C, Ohlsson K
机构信息
Department of Surgery, University of Lund, Malmö General Hospital, Sweden.
出版信息
J Clin Pathol. 1992 Dec;45(12):1066-9. doi: 10.1136/jcp.45.12.1066.
AIMS
To measure the content of immunoreactive human pancreatic secretory trypsin inhibitor (irPSTI) in colonic carcinoma and adjacent normal colonic mucosa.
METHODS
From a stable hybridoma cell line producing monoclonal antibodies specific for human PSTI, a specific enzyme linked immunosorbent assay (ELISA) for human PSTI was developed. In a precipitation assay system these antibodies bound human PSTI in a dose-dependent manner. The specimens were obtained from resectional surgery.
RESULTS
The content of irPSTI was 19.9 micrograms/g protein (0.55 micrograms/g tissue wet weight) in colonic carcinoma. In adjacent normal colonic mucosa 43.6 micrograms/g protein (1.12 micrograms/g tissue wet weight) was shown.
CONCLUSIONS
The enzymatic degradation of surrounding tissue necessary for tumour cell invasion could be facilitated by this relative deficit of the inhibitor in infiltrative carcinoma.
目的
检测结肠癌及相邻正常结肠黏膜中免疫反应性人胰腺分泌性胰蛋白酶抑制剂（irPSTI）的含量。
方法
利用产生针对人PSTI特异性单克隆抗体的稳定杂交瘤细胞系，开发了一种针对人PSTI的特异性酶联免疫吸附测定（ELISA）。在沉淀测定系统中，这些抗体以剂量依赖方式与人PSTI结合。标本取自手术切除组织。
结果
结肠癌中irPSTI的含量为19.9微克/克蛋白质（0.55微克/克组织湿重）。相邻正常结肠黏膜中显示为43.6微克/克蛋白质（1.12微克/克组织湿重）。
结论
浸润性癌中这种抑制剂的相对缺乏可能会促进肿瘤细胞侵袭所需的周围组织的酶解降解。
Zotero 插件