Marques M E, Coelho K I, Sotto M N, Bacchi C E
Department of Pathology, Botucatu Medical School-UNESP, Botucatu, São Paulo, Brazil.
J Clin Pathol. 1992 Dec;45(12):1089-93. doi: 10.1136/jcp.45.12.1089.
To compare the efficacy of histochemical and immunohistochemical methods in detecting forms of Sporothrix schenckii in tissue.
Thirty five cutaneous biopsy specimens from 27 patients with sporotrichosis were stained by histochemical haematoxylin and eosin, periodic acid Schiff, and Gomori's methenamine silver methods and an immunohistochemical (avidin-biotin complex immunoperoxidase) (ABC) technique associated with a newly produced rabbit polyclonal antibody anti-Sporothrix schenckii.
A total of 29 (83%) cases were positive by the ABC method used in association with anti-Sporothrix schenckii rabbit polyclonal antibodies. Histochemical methods, using silver staining, periodic acid Schiff, and conventional haematoxylin and eosin detected 37%, 23%, and 23% of forms of S schenckii, respectively. The ABC technique was significantly more reliable than periodic acid Schiff and silver staining techniques.
It is concluded that immunostaining is an easy and rapid method which can efficiently increase the accuracy of the diagnosis of sporotrichosis in human tissue.
比较组织化学和免疫组织化学方法检测组织中申克孢子丝菌形态的效果。
对27例孢子丝菌病患者的35份皮肤活检标本进行苏木精-伊红组织化学染色、过碘酸希夫染色、Gomori六胺银染色，并采用与新制备的兔抗申克孢子丝菌多克隆抗体相关的免疫组织化学（抗生物素蛋白-生物素复合物免疫过氧化物酶）（ABC）技术。
与兔抗申克孢子丝菌多克隆抗体联合使用的ABC方法检测出29例（83%）阳性病例。采用银染色、过碘酸希夫染色和传统苏木精-伊红组织化学方法分别检测出申克孢子丝菌形态的比例为37%、23%和23%。ABC技术明显比过碘酸希夫染色和银染色技术更可靠。
得出结论，免疫染色是一种简便快速的方法，能有效提高人体组织中孢子丝菌病诊断的准确性。
