de Miranda L H M, Quintella L P, dos Santos I B, Menezes R C, Figueiredo F B, Gremião I D F, Okamoto T, de Oliveira R V C, Pereira S A, Tortelly R, Schubach T M P
Instituto de Pesquisa Clínica Evandro Chagas, Fundação Oswaldo Cruz, Rio de Janeiro, RJ, Brazil.
Mycopathologia. 2009 Aug;168(2):79-87. doi: 10.1007/s11046-009-9198-4. Epub 2009 Apr 10.
The present study reports the histopathological findings of 86 skin lesions of dogs with sporotrichosis from Rio de Janeiro. Suppurative granulomatous inflammation was the predominant finding and was observed in 76 (88.37%) cases. Plasma cells surrounding the suppurative granulomas were detected in 68 (89.5%) cases and an inflammatory infiltrate at the periphery of these granulomatous lesions was observed in 63 (82.9%). Fungus-specific staining revealed yeast cells compatible with Sporothrix schenckii in 36 cases. These fungal elements were only detected in lesions characterized by suppurative granulomatous inflammation. Thus, specific staining of serial sections is recommended in the case of dogs with skin lesions whose histopathological presentation is consistent with sporotrichosis. However, due to the generally small number of yeast cells in lesions, the hypothesis of sporotrichosis should not be ruled out even if the result is negative, especially in epidemic areas where correlation with epidemiological data is particularly useful.
本研究报告了来自里约热内卢的86例患有孢子丝菌病的犬皮肤病变的组织病理学检查结果。化脓性肉芽肿性炎症是主要发现，在76例（88.37%）病例中观察到。68例（89.5%）病例中检测到围绕化脓性肉芽肿的浆细胞，63例（82.9%）病例中观察到这些肉芽肿性病变周边的炎性浸润。真菌特异性染色在36例中显示与申克孢子丝菌相符的酵母细胞。这些真菌成分仅在以化脓性肉芽肿性炎症为特征的病变中检测到。因此，对于组织病理学表现与孢子丝菌病一致的皮肤病变犬，建议对连续切片进行特异性染色。然而，由于病变中酵母细胞数量通常较少，即使结果为阴性，孢子丝菌病的可能性也不应排除，特别是在与流行病学数据相关特别有用的流行地区。
