Laboratório de Pesquisa Clínica em Dermatozoonoses em Animais Domésticos (LAPCLIN-DERMZOO), Instituto de Pesquisa Clínica Evandro Chagas, Fundação Oswaldo Cruz (IPEC, FIOCRUZ), Av. Brasil, 436, Manguinhos, 21040-360 Rio de Janeiro, RJ, Brazil.
Vet J. 2011 Dec;190(3):408-11. doi: 10.1016/j.tvjl.2010.12.004. Epub 2011 Jan 8.
The aim of this study was to apply immunohistochemistry (IHC) for the diagnosis of canine sporotrichosis and to compare this method with the Grocott's silver stain (GSS) and periodic acid Schiff (PAS) techniques. Eighty-seven dogs with sporotrichosis (group 1) and 35 with American tegumentary leishmaniosis (ATL) (group 2) were studied. The fungus was detected in group 1 by GSS, PAS and IHC. IHC was also applied to group 2 to evaluate the occurrence of cross-reactions. PAS, GSS and IHC detected yeast cells in 19.5%, 43.7% and 65.5% of the group 1 cases, respectively. The detection of intracellular antigens of Sporothrix schenckii by IHC increased the sensitivity of the histological diagnosis to 80.5%. No positive reaction was observed in ATL lesions. The results suggest that IHC may be indicated for the diagnosis of sporotrichosis because of its higher diagnostic sensitivity.
本研究旨在应用免疫组织化学（IHC）诊断犬孢子丝菌病，并将该方法与 Grocott 银染色（GSS）和过碘酸希夫（PAS）技术进行比较。研究了 87 例孢子丝菌病（第 1 组）和 35 例美洲皮肤利什曼病（ATL）（第 2 组）的犬。第 1 组通过 GSS、PAS 和 IHC 检测到真菌。IHC 还应用于第 2 组以评估交叉反应的发生。PAS、GSS 和 IHC 在第 1 组的 19.5%、43.7%和 65.5%的病例中分别检测到酵母细胞。IHC 检测申克孢子丝菌的细胞内抗原将组织学诊断的敏感性提高到 80.5%。在 ATL 病变中未观察到阳性反应。结果表明，IHC 可能是孢子丝菌病诊断的指征，因为它具有更高的诊断敏感性。
