Davis K, Bottone E J, Lucas D, Lebwohl M
Department of Dermatology, Mount Sinai Medical Center, New York, NY.
J Am Acad Dermatol. 1992 Dec;27(6 Pt 1):959-61. doi: 10.1016/0190-9622(92)70294-p.
Injectable collagen has been used for more than 15 years to correct soft tissue cosmetic defects. After injection, the collagen remaining in the syringe is often refrigerated for later use in the same patient, despite manufacturer and Food and Drug Administration recommendations to discard the unused collagen.
This study examined the incidence of bacterial contamination of stored collagen.
Syringe needle tips and collagen from 50 previously used syringes containing either Zyderm I, Zyderm II, or Zyplast were cultured separately under aerobic and anaerobic conditions. Bacterial isolates were identified.
Propionibacterium acnes was cultured from 7 of 50 needle tips. One positive needle tip culture grew both P. acnes and Staphylococcus aureus. Bacteria were isolated from only one collagen sample that grew a nonhemolytic streptococcus that may have represented a laboratory contaminant.
Syringes of collagen stored for repeated use rarely become contaminated with bacteria despite frequent contamination of their needle tips. Skin abscesses after collagen injection should be cultured under anaerobic, as well as aerobic, conditions so that infections caused by P. acnes will not be missed.
可注射胶原蛋白已用于矫正软组织美容缺陷超过15年。注射后，注射器中剩余的胶原蛋白常被冷藏以便在同一患者后续使用，尽管制造商和食品药品监督管理局建议丢弃未使用的胶原蛋白。
本研究检测储存胶原蛋白的细菌污染发生率。
对50个先前使用过的装有Zyderm I、Zyderm II或Zyplast的注射器的针头尖端和胶原蛋白分别在需氧和厌氧条件下进行培养。鉴定细菌分离株。
从50个针头尖端中的7个培养出痤疮丙酸杆菌。一个阳性针头尖端培养物中同时生长出痤疮丙酸杆菌和金黄色葡萄球菌。仅从一个胶原蛋白样本中分离出细菌，该样本生长出一种可能代表实验室污染物的非溶血性链球菌。
尽管胶原蛋白注射器的针头尖端经常被污染，但储存以供重复使用的胶原蛋白注射器很少被细菌污染。胶原蛋白注射后出现的皮肤脓肿应在需氧和厌氧条件下进行培养，以免漏诊由痤疮丙酸杆菌引起的感染。
