向海兔B型光感受器内注射BAPTA可阻断增强作用的诱导。

Intracellular injections of BAPTA block induction of enhancement in Hermissenda type B-photoreceptors.

作者信息

Falk-Vairant J, Crow T

机构信息

Department of Neurobiology and Anatomy, University of Texas Medical School, Houston 77225.

出版信息

Neurosci Lett. 1992 Nov 23;147(1):45-8. doi: 10.1016/0304-3940(92)90771-x.

DOI:10.1016/0304-3940(92)90771-x

PMID:1480322

Abstract

Light paired with serotonin (5-HT) in vivo produces both short- and long-term enhancement of generator potentials in identified B-photoreceptors in Hermissenda. The role of intracellular Ca2+ in the induction of enhancement in B-photoreceptors was assessed by buffering intracellular Ca2+ with the iontophoretic injection of BAPTA. Blocking light-elicited photoreceptor desensitization with BAPTA loading prior to applying 5-HT was used as an indication of the effectiveness of Ca2+ buffering. Enhancement was blocked in preparations that received BAPTA loading prior to the application of 5-HT while typical enhancement was elicited by light and 5-HT in control preparations. These results indicate that enhancement involves a Ca(2+)-dependent process.

摘要

在体内，光与血清素（5-羟色胺，5-HT）相结合，会使海兔（Hermissenda）中已识别的B型光感受器的发生器电位产生短期和长期增强。通过离子电渗注入BAPTA缓冲细胞内Ca2+，评估细胞内Ca2+在B型光感受器增强诱导中的作用。在应用5-HT之前用BAPTA负载来阻断光引发的光感受器脱敏，以此作为Ca2+缓冲有效性的指标。在应用5-HT之前接受BAPTA负载的制剂中，增强作用被阻断，而在对照制剂中，光和5-HT引发了典型的增强作用。这些结果表明，增强作用涉及一个Ca(2+)依赖性过程。