Crow T, Forrester J, Williams M, Waxham M N, Neary J T
Department of Neurobiology and Anatomy, University of Texas Medical School, Houston 77225.
Neurosci Lett. 1991 Jan 2;121(1-2):107-10. doi: 10.1016/0304-3940(91)90660-l.
Light paired with serotonin (5-HT) in vivo produces both short and long-term enhancement of generator potentials in identified B-photoreceptors in Hermissenda. The contribution of protein kinase C to the induction of enhancement was assessed by pretreatment with 12-O-tetradecanoyl-phorbol-13-acetate (TPA), which produces a depletion or down-regulation of protein kinase C. Presentation of light and 5-HT after an 8 h pretreatment with TPA blocked the induction of enhancement of light-evoked generator potentials. Typical enhancement produced by light and 5-HT was observed after pretreatment with an inactive phorbol (4 alpha-phorbol). These results indicate that activation of protein kinase C is an important step in the induction of enhancement.
在体内，光与血清素（5-羟色胺，5-HT）相结合，可使海兔中已识别的B型光感受器的发生器电位产生短期和长期增强。通过用12-O-十四烷酰佛波醇-13-乙酸酯（TPA）进行预处理来评估蛋白激酶C对增强诱导的作用，TPA会导致蛋白激酶C的消耗或下调。在用TPA预处理8小时后再呈现光和5-HT，会阻断光诱发的发生器电位增强的诱导。在用无活性佛波醇（4α-佛波醇）预处理后，观察到了由光和5-HT产生的典型增强。这些结果表明，蛋白激酶C的激活是增强诱导过程中的一个重要步骤。
