Crow T, Forrester J
Department of Physiology, School of Medicine, University of Pittsburgh, PA 15261.
Proc Natl Acad Sci U S A. 1990 Jun;87(12):4490-4. doi: 10.1073/pnas.87.12.4490.
A one-trial in vivo conditioning procedure produces short- and long-term cellular changes that can be detected in identified sensory neurons of the pathway mediating the conditioned stimulus. The memory of the associative experience in the conditioned stimulus pathway is expressed by short- and long-term enhancement of light-evoked generator potentials recorded from identified lateral and medial type B photoreceptors within the eyes of Hermissenda. To identify mechanisms of the induction and expression of short- and long-term enhancement in identified photoreceptors, we have investigated the effects of inhibiting protein synthesis during the application of the one-trial in vivo conditioning procedure. Anisomycin (1 microM) present during and after the conditioning trial blocked long-term enhancement without affecting the induction or expression of short-term enhancement. Application of a control compound, deacetylanisomycin (1 microM), or delaying the application of anisomycin until 1 hr after the conditioning trial did not block either long- or short-term enhancement. These results indicate that synthesis of proteins during or shortly after training may be a critical step in the formation of long-term memory of the associative experience.
单次体内条件作用程序会产生短期和长期的细胞变化，这些变化可在介导条件刺激的通路中已确定的感觉神经元中检测到。在条件刺激通路中，联想经验的记忆通过从Hermissenda眼睛中已确定的外侧和内侧B型光感受器记录的光诱发发生器电位的短期和长期增强来表达。为了确定已确定的光感受器中短期和长期增强的诱导和表达机制，我们研究了在单次体内条件作用程序应用期间抑制蛋白质合成的影响。在条件作用试验期间及之后存在的茴香霉素（1 microM）阻断了长期增强，而不影响短期增强的诱导或表达。应用对照化合物脱乙酰茴香霉素（1 microM），或将茴香霉素的应用延迟到条件作用试验后1小时，均未阻断长期或短期增强。这些结果表明，训练期间或之后不久蛋白质的合成可能是联想经验长期记忆形成中的关键步骤。
