Siragusa G R
Roman L. Hruska U.S. Meat Animal Research Center, U.S. Department of Agriculture, Clay Center, Nebraska 68933.
Appl Environ Microbiol. 1992 Nov;58(11):3508-13. doi: 10.1128/aem.58.11.3508-3513.1992.
A bovine intestinal bacterial isolate, identified as Enterococcus hirae, was found to produce a bacteriocin (designated hiraecin S) inhibitory to Listeria monocytogenes and other Listeria spp. Identification to species level was determined by comprehensive biochemical and morphological tests which were verified by DNA-DNA homology assays. The antimicrobial agent was inactivated by pronase and papain and was insensitive to catalase. The antimicrobial activity was not due to hydrogen peroxide or acid formation, nor was lysozyme or muramidase activity observed in cell-free bacteriocin preparations. Inhibition of selected gram-negative bacteria was not observed. Other enterococci were sensitive to the bacteriocin, and except for Listeria spp., no other gram-positive bacteria tested were inhibited.
一种被鉴定为平肠球菌的牛肠道细菌分离株,被发现能产生一种对单核细胞增生李斯特菌和其他李斯特菌属有抑制作用的细菌素(命名为希拉菌素S)。通过全面的生化和形态学测试确定到种水平,并通过DNA-DNA同源性分析进行验证。该抗菌剂被链霉蛋白酶和木瓜蛋白酶灭活,对过氧化氢酶不敏感。抗菌活性不是由于过氧化氢或酸的形成,在无细胞细菌素制剂中也未观察到溶菌酶或胞壁质酶活性。未观察到对选定革兰氏阴性菌的抑制作用。其他肠球菌对该细菌素敏感,除李斯特菌属外,测试的其他革兰氏阳性菌均未被抑制。