Facklam R R, Collins M D
Division of Bacterial Diseases, Centers for Disease Control, Atlanta, Georgia 30333.
J Clin Microbiol. 1989 Apr;27(4):731-4. doi: 10.1128/jcm.27.4.731-734.1989.
Streptococci (206 cultures) previously identified as enterococci were retrieved from storage and reidentified by using tests designed to identify species of the genus Enterococcus. Of these 188, 91% were correctly identified as Enterococcus species. Of the remaining strains, nine (4%) were unidentified and six (3%) and 3 (1.5%) were identified as Leuconostoc sp. and Lactococcus sp., respectively. Two new Enterococcus species were discovered: E. raffinosus and E. solitarius. DNA-DNA hybridizations were performed on selected strains to assure correct identification. Cultures representing 10 of the 12 Enterococcus species were among the 188 strains identified. An identification system based on the grouping of key reactions of 20 phenotypic characteristics of Enterococcus species is described.
从保藏物中取出先前鉴定为肠球菌的链球菌(206株培养物),并使用旨在鉴定肠球菌属物种的试验进行重新鉴定。在这188株中,91%被正确鉴定为肠球菌属物种。其余菌株中,9株(4%)未鉴定出来,6株(3%)和3株(1.5%)分别被鉴定为明串珠菌属和乳球菌属。发现了两个新的肠球菌物种:棉子糖肠球菌和孤独肠球菌。对选定菌株进行了DNA-DNA杂交以确保正确鉴定。在鉴定出的188株菌株中,有代表12种肠球菌中10种的培养物。描述了一种基于肠球菌属20个表型特征关键反应分组的鉴定系统。