Iwasaki E, Baba M
Department of Pediatrics, Doai Memorial Hospital.
Arerugi. 1992 Oct;41(10):1449-58.
Classification and characterization of allergens is important because allergic patients are sensitized by a variety of allergens. One hundred and sixty-one sera from asthmatic children were investigated for specific IgE antibodies against 35 allergens including 20 inhalants and 15 foods by means of the MAST method. We assessed the allergenic properties of the allergens based on positive percentage agreement and cluster analysis. There was a high positive percentage agreement of specific IgE antibodies between house dust and Dermatophagoides spp., a relatively high agreement between 5 molds, cat and dog epithelium, mugwort and wormwood and 5 grasses. Among the food allergens, the positive percentage agreements were relatively high, especially between cow's milk, casein, cheese, and between 3 cereal grains. In the cluster analysis, house dust and Dermatophagoides spp. made a big cluster; therefore 32 allergens except house dust and mites were analyzed. From the results of the cluster analysis, the major cluster consisted of (1) ragweed, (2) mugwort and wormwood, (3) timothy, sweet vernal, velvet and cultivated rye, (4) wheat, barley and rice, (5) molds, (6) cow's milk, casein, soybean and cheese, (7) shrimp and crab, (8) egg white, (9) Japanese cedar, (10) dog epithelium, (11) cat epithelium. The cluster of grass pollens and cereal grains made one cluster. These results tend to confirm the presence of species cross-reactivities within the major classes of allergens.
过敏原的分类与特性鉴定十分重要，因为过敏患者会被多种过敏原致敏。采用MAST方法，对161份哮喘儿童血清进行检测，以分析其针对35种过敏原（包括20种吸入性过敏原和15种食物过敏原）的特异性IgE抗体。我们基于阳性百分比一致性和聚类分析评估了过敏原的致敏特性。屋尘与粉尘螨之间特异性IgE抗体的阳性百分比一致性较高，5种霉菌、猫和狗上皮、艾蒿和青蒿以及5种草之间的一致性相对较高。在食物过敏原中，阳性百分比一致性相对较高，尤其是牛奶、酪蛋白、奶酪之间，以及3种谷物之间。在聚类分析中，屋尘和粉尘螨聚为一大类；因此，对除屋尘和螨虫之外的32种过敏原进行了分析。根据聚类分析结果，主要类别包括：（1）豚草，（2）艾蒿和青蒿，（3）梯牧草、春黄菊、绒毛草和栽培黑麦，（4）小麦、大麦和水稻，（5）霉菌，（6）牛奶、酪蛋白、大豆和奶酪，（7）虾和蟹，（8）蛋清，（9）日本柳杉，（10）狗上皮，（11）猫上皮。草花粉和谷物聚为一类。这些结果倾向于证实主要过敏原类别中存在物种交叉反应性。
