Kawamoto T, Kishimoto K, Takahashi K, Matsumura T, Sato J D, Taniguchi S
Department of Biochemistry, Okayama University Dental School, Japan.
In Vitro Cell Dev Biol. 1992 Nov-Dec;28A(11-12):782-6. doi: 10.1007/BF02631068.
The ability of different Fc receptors (Fc gamma R) on IgG-mediated cytotoxicity for human epidermoid carcinoma A431 cells bearing large number of epidermal growth factor receptors (EGFRs) was examined by using two isotypes (IgG1 and IgG2a) of murine monoclonal antibodies (MoAbs) against EGFR in the presence of human monocytes and granulocytes. Two MoAbs (225 and LA1) of the IgG1 isotype exhibited effective cytolytic activity for A431 cells with human polymorphonuclear leukocytes (PMN) rather than with human mononuclear cells (MNC). In contrast, two MoAbs (528 and 579) of the IgG2a-isotype lysed the cells less effectively with PMN than with MNC. Anti-Fc gamma R II (CDw32) MoAb 2E1 inhibited the IgG1-mediated cytotoxicity by PMN, and anti-Fc gamma R III (CD16) MoAb 80H3 did not inhibit the IgG2a-mediated cytotoxicity by MNC. Under these conditions, antibody-dependent cell-mediated cytotoxicity by mouse MoAb IgG1 isotypes resulted from antibody binding to the Fc gamma R II (CDw32) of PMN.
在人单核细胞和粒细胞存在的情况下，使用两种针对表皮生长因子受体（EGFR）的鼠单克隆抗体（MoAb）同型（IgG1和IgG2a），检测了不同Fc受体（FcγR）对携带大量EGFR的人表皮样癌A431细胞的IgG介导的细胞毒性作用。IgG1同型的两种MoAb（225和LA1）对A431细胞与人多形核白细胞（PMN）而非人单核细胞（MNC）一起时表现出有效的溶细胞活性。相反，IgG2a同型的两种MoAb（528和579）与PMN一起时对细胞的裂解效果不如与MNC一起时有效。抗FcγR II（CDw32）MoAb 2E1抑制了PMN介导的IgG1细胞毒性，而抗FcγR III（CD16）MoAb 80H3不抑制MNC介导的IgG2a细胞毒性。在这些条件下，小鼠MoAb IgG1同型的抗体依赖性细胞介导的细胞毒性是由于抗体与PMN的FcγR II（CDw32）结合所致。
