Vencovsky J, Williams D G, Field M, Maini R N
Kennedy Institute of Rheumatology, London, United Kingdom.
Ann Rheum Dis. 1992 Dec;51(12):1313-7. doi: 10.1136/ard.51.12.1313.
The ribonucleoprotein (RNP) p67 antigen was purified from rabbit thymus and used in an enzyme linked immunosorbent assay (ELISA) with low interassay variability to detect IgG antibodies to p67 in patients with autoimmune connective tissue diseases. These antibodies were found in eight (80%) patients with a clinical diagnosis of mixed connective tissue disease (MCTD) but also in 27 (40%) patients with systemic lupus erythematosus (SLE). Sixty six per cent of the 12 patients with SLE with high levels of antibodies to p67 (> 50 U) had three or more features of MCTD, including myositis, fibrosing alveolitis, Raynaud's phenomenon, and sclerodactyly. Antibodies to the p67 RNP were not associated with the presence or absence of renal disease in the patients with SLE. This study suggests that antibodies against the p67 RNP are markers for clinical features of MCTD even in the context of SLE.
核糖核蛋白（RNP）p67抗原从兔胸腺中纯化出来，并用于酶联免疫吸附测定（ELISA），该测定法具有较低的批间变异性，用于检测自身免疫性结缔组织病患者中针对p67的IgG抗体。这些抗体在临床诊断为混合性结缔组织病（MCTD）的8名（80%）患者中被发现，但也在27名（40%）系统性红斑狼疮（SLE）患者中被发现。在12名p67抗体水平较高（>50 U）的SLE患者中，66%具有三种或更多MCTD特征，包括肌炎、纤维化肺泡炎、雷诺现象和指端硬化。在SLE患者中，针对p67 RNP的抗体与是否存在肾脏疾病无关。这项研究表明，即使在SLE的背景下，针对p67 RNP的抗体也是MCTD临床特征的标志物。
