Bernard A, Marchandise F X, Depelchin S, Lauwerys R, Sibille Y
Industrial Toxicology and Occupational Medicine Unit, Catholic University of Louvain, Brussels, Belgium.
Eur Respir J. 1992 Nov;5(10):1231-8.
The 10 kDa Clara cell protein was measured in serum and bronchoalveolar lavage (BAL) from 39 healthy subjects (14 smokers, 25 nonsmokers) and from 41 patients with respiratory disease (chronic obstructive pulmonary disease (COPD), sarcoidosis, lung cancer). Clara cell protein appears as one of the most abundant respiratory tract derived proteins, with values averaging 7% of the total protein content of lung lavages from healthy nonsmokers. A significant reduction of Clara cell protein was found in BAL from smokers and patients with COPD or lung cancer. The same pattern of change was found in the concentrations of Clara cell protein in serum. Pulmonary sarcoidosis did not affect absolute values of Clara cell protein in lung lavages but was associated with elevated levels in serum. Changes in lung lavage Clara cell protein differed from that of albumin, beta 2-microglobulin or the secretory component, since the latter were unaffected by smoking or COPD but increased in sarcoidosis and lung cancer. These results indicate that Clara cell protein in BAL or serum might serve as a sensitive indicator of nonciliated bronchial cell dysfunction.
在39名健康受试者（14名吸烟者，25名非吸烟者）以及41名患有呼吸系统疾病（慢性阻塞性肺疾病（COPD）、结节病、肺癌）的患者的血清和支气管肺泡灌洗（BAL）液中检测了10 kDa克拉拉细胞蛋白。克拉拉细胞蛋白似乎是最丰富的呼吸道衍生蛋白之一，在健康非吸烟者的肺灌洗液中，其含量平均占总蛋白含量的7%。在吸烟者以及患有COPD或肺癌的患者的BAL液中，发现克拉拉细胞蛋白显著减少。血清中克拉拉细胞蛋白的浓度也呈现出相同的变化模式。肺部结节病并未影响肺灌洗液中克拉拉细胞蛋白的绝对值，但与血清中该蛋白水平升高有关。肺灌洗克拉拉细胞蛋白的变化与白蛋白、β2-微球蛋白或分泌成分不同，因为后者不受吸烟或COPD的影响，但在结节病和肺癌中会增加。这些结果表明，BAL液或血清中的克拉拉细胞蛋白可能是无纤毛支气管细胞功能障碍的敏感指标。
