Department of Pharmacy, University "G. d'Annunzio", Chieti-Pescara, Via dei Vestini, 31, Chieti, Italy.
J Mater Sci Mater Med. 2013 Aug;24(8):1977-83. doi: 10.1007/s10856-013-4949-7. Epub 2013 May 14.
This study aimed to investigate the effect of saliva on Streptococcus mitis free cells and on S. mitis/human gingival fibroblasts (HGFs) co-culture model, in presence of 2-hydroxyethyl-methacrylate (HEMA). The bacterial aggregation both in the planktonic phase and on HGFs, as well as the apoptotic and necrotic eukaryotic cells amount were analyzed, in presence of saliva and/or HEMA. The aggregation test revealed a significant saliva aggregation effect on S. mitis strains compared to the untreated sample. No significant differences were recorded in the amount of culturable bacteria in all studied conditions; however, from microscopy images, the saliva/HEMA combining effect induced a significant bacterial aggregation and adhesion on HGFs. HEMA treatment decreased viable eukaryotic cell number with a parallel increment of necrotic cells, but when saliva was added to the co-culture, the viable cells percentage increased to a value comparable to the control sample.
本研究旨在探讨唾液对游离变异链球菌和变异链球菌/人牙龈成纤维细胞（HGFs）共培养模型的影响，同时还研究了 2-羟乙基甲基丙烯酸酯（HEMA）的存在对这些模型的影响。分析了在唾液和/或 HEMA 存在的情况下，细菌在浮游期和 HGFs 上的聚集情况，以及真核细胞凋亡和坏死的数量。聚集试验显示，与未经处理的样本相比，唾液对变异链球菌菌株有明显的聚集作用。在所有研究条件下，可培养细菌的数量均无显著差异；然而，从显微镜图像可以看出，唾液/HEMA 的联合作用导致细菌在 HGFs 上发生明显的聚集和黏附。HEMA 处理会降低活真核细胞的数量，同时增加坏死细胞的数量，但当向共培养物中添加唾液时，活细胞的百分比增加到与对照样本相当的值。
