Sjöstrand J, Karlsson J O, Andersson A K
Department of Ophthalmology, University of Gothenburg, Sweden.
Acta Ophthalmol (Copenh). 1992 Dec;70(6):814-9. doi: 10.1111/j.1755-3768.1992.tb04893.x.
Samples of the vitreous were analysed in order to identify changes of soluble proteins in vitreo-retinal disease. The soluble proteins of the vitreous were separated on an anion exchange column (Mono-Q). The degree of neutral proteolytic activity in vitreous body was also measured. The vitreous from cataract cases without vitreoretinal disease was characterized by its low content of soluble proteins equivalent to about 1% of that of serum. Albumin and transferrin were the major identified components and their concentrations were approximately 0.85 and 0.03 g/l, respectively. In cases with vitreoretinal disease the vitreous showed changes of total soluble protein and the appearance of additional protein peaks. In patients with PVR the albumin concentration in the vitreous was found to be three times higher as compared to the control group consisting of patients with cataract. Neutral proteolytic activity in the vitreous was relatively low in both normal and pathological vitreous.
为了确定玻璃体视网膜疾病中可溶性蛋白质的变化，对玻璃体样本进行了分析。玻璃体中的可溶性蛋白质在阴离子交换柱（Mono-Q）上进行分离。还测量了玻璃体中中性蛋白水解活性。无玻璃体视网膜疾病的白内障病例的玻璃体，其特征在于可溶性蛋白质含量低，约相当于血清含量的1%。白蛋白和转铁蛋白是主要鉴定出的成分，其浓度分别约为0.85 g/l和0.03 g/l。在玻璃体视网膜疾病病例中，玻璃体显示出总可溶性蛋白质的变化以及额外蛋白质峰的出现。在增殖性玻璃体视网膜病变（PVR）患者中，发现玻璃体中的白蛋白浓度比由白内障患者组成的对照组高三倍。正常和病理玻璃体中的中性蛋白水解活性都相对较低。
