Banerjee Somnath, Savant Vijay, Scott Robert A H, Curnow S John, Wallace Graham R, Murray Philip I
Academic Unit of Ophthalmology, Division of Immunity and Infection, University of Birmingham, Birmingham, United Kingdom.
Invest Ophthalmol Vis Sci. 2007 May;48(5):2203-7. doi: 10.1167/iovs.06-1358.
Vitreoretinal disorders are frequently characterized by increased vitreous levels of cellular mediators, including cytokines, chemokines, and growth factors. The study was conducted to investigate whether multiplex bead analysis could identify disease-specific profiles of these mediators in a variety of vitreoretinal diseases.
Levels of 19 mediators were measured: the cytokines IL-6, IL-10, IL-12, IL-13, IL-15, IL-17, TNF, IFN-gamma, granulocyte-macrophage-colony-stimulating factor (GM-CSF), and granulocyte-stimulating factor (G-CSF); the chemokines CCL2, CCL3, CCL4, CCL5, CCL11, and CXCL8; and the growth factors epidermal growth factor (EGF), FGF, and VEGF, by using multiplex bead analysis of vitreous humor of 58 eyes undergoing vitrectomy for a variety of vitreoretinal disorders.
The predominant mediators detected were IL-6, CXCL8, and CCL2. The most complex pattern of mediators was seen in patients with proliferative vitreoretinopathy (PVR) and included a mixture of cytokines, chemokines, and growth factors. Patients with chronic uveitis showed a limited mediator pattern that did not suggest either a Th1 or Th2 response. By comparison, patients with lens-induced uveitis (LIU) showed significantly greater levels of cytokines than did patients with chronic uveitis, including IFN-gamma and IL-12, with a trend toward an acute Th1 inflammatory response. Moreover, in samples from patients with LIU, CXCL8 inversely correlated with time after initial surgery and duration of treatment.
Multiplex bead analysis allows the measurement of multiple mediators from a single vitreous sample. The data confirm patterns of mediators previously described in different vitreoretinal conditions. In addition, LIU mediator levels correlate with duration of treatment and time after cataract surgery.
玻璃体视网膜疾病的特征通常是玻璃体中细胞介质水平升高，包括细胞因子、趋化因子和生长因子。本研究旨在调查多重微珠分析是否能够识别多种玻璃体视网膜疾病中这些介质的疾病特异性谱。
通过对58只因各种玻璃体视网膜疾病接受玻璃体切除术的眼睛的玻璃体液进行多重微珠分析，测量19种介质的水平：细胞因子白细胞介素-6（IL-6）、白细胞介素-10（IL-10）、白细胞介素-12（IL-12）、白细胞介素-13（IL-13）、白细胞介素-15（IL-15）、白细胞介素-17（IL-17）、肿瘤坏死因子（TNF）、干扰素-γ（IFN-γ）、粒细胞-巨噬细胞集落刺激因子（GM-CSF）和粒细胞刺激因子（G-CSF）；趋化因子CCL2、CCL3、CCL4、CCL5、CCL11和CXCL8；以及生长因子表皮生长因子（EGF）、成纤维细胞生长因子（FGF）和血管内皮生长因子（VEGF）。
检测到的主要介质为IL-6、CXCL8和CCL2。在增殖性玻璃体视网膜病变（PVR）患者中观察到最复杂的介质模式，包括细胞因子、趋化因子和生长因子的混合。慢性葡萄膜炎患者显示出有限的介质模式，未提示Th1或Th2反应。相比之下，晶状体诱导性葡萄膜炎（LIU）患者的细胞因子水平显著高于慢性葡萄膜炎患者，包括IFN-γ和IL-12，有急性Th1炎症反应的趋势。此外，在LIU患者的样本中，CXCL8与初次手术后的时间和治疗持续时间呈负相关。
多重微珠分析可从单个玻璃体液样本中测量多种介质。数据证实了先前在不同玻璃体视网膜疾病中描述的介质模式。此外，LIU的介质水平与治疗持续时间和白内障手术后的时间相关。
