Fenton R A, Dobson J G
Department of Physiology, University of Massachusetts Medical School, Worcester 01655.
Anal Biochem. 1992 Nov 15;207(1):134-41. doi: 10.1016/0003-2697(92)90514-8.
Adenosine is a naturally occurring nucleoside which regulates many physiological processes by interacting with adenosine-specific receptors. Knowledge of the extracellular adenosine concentration at the site of adenosine receptors on target cells is required for an understanding of mechanisms involving the action of the nucleoside. Samples of extracellular fluid which reside in close proximity to the surface of target cells are frequently small in volume. This report describes improvements in accuracy and reliability of a fluorometric assay designed for determining the concentration of adenosine in microliter samples of extracellular fluids. The utility of the assay is demonstrated by determining adenosine concentrations in interstitial and coronary effluent samples from normoxic perfused rat hearts. The assay also clearly detects changes in the interstitial and coronary effluent adenosine levels produced by isoproterenol stimulation or hypoxia. Thus, this assay is useful for determining the adenosine concentration in microliter samples of extracellular fluid and should facilitate investigations dealing with the functions of adenosine.
腺苷是一种天然存在的核苷，它通过与腺苷特异性受体相互作用来调节许多生理过程。要了解涉及该核苷作用的机制，就需要知道靶细胞上腺苷受体部位的细胞外腺苷浓度。紧邻靶细胞表面的细胞外液样本体积通常很小。本报告描述了一种荧光测定法在准确性和可靠性方面的改进，该方法用于测定微升体积的细胞外液样本中腺苷的浓度。通过测定常氧灌注大鼠心脏的间质液和冠状流出液样本中的腺苷浓度，证明了该测定法的实用性。该测定法还能清晰地检测到异丙肾上腺素刺激或缺氧引起的间质液和冠状流出液中腺苷水平的变化。因此，该测定法可用于测定微升体积的细胞外液样本中的腺苷浓度，应该有助于开展有关腺苷功能的研究。
