Hassen A, Hachicha R, Jedidi N, Agbalika F, Harteman P
Institut National de Recherche Scientifique et Technique (INRST), Vandoeuvre, Nancy.
Arch Inst Pasteur Tunis. 1992 Jan-Apr;69(1-2):13-9.
The objective of this study was the amelioration of the organic acid precipitation technique currently used as a secondary stage for viruses concentration using ferric chloride (FeCl3) as an adjuvant. To carry out this work successfully, we have studied the cytotoxicity effect of FeCl3 towards cell lines usually used for the investigation of enteric viruses. Two kinds of african green monkey kidney cells, MA104 and BGM, were used as a cellular model. The results showed that optimum concentration of FeCl3 for either BGM or MA104 cell survival and organic acid precipitation varies from 0.5 to 1 mM. The cytotoxicity test used in this work was simple, easy to realize and gives an appreciable information about the cytotoxicity dose of a given biological and chemical product.
本研究的目的是改进目前作为病毒浓缩第二阶段使用的有机酸沉淀技术，该技术使用氯化铁（FeCl3）作为佐剂。为了成功开展这项工作，我们研究了FeCl3对通常用于肠道病毒研究的细胞系的细胞毒性作用。两种非洲绿猴肾细胞，MA104和BGM，被用作细胞模型。结果表明，对于BGM或MA104细胞存活和有机酸沉淀而言，FeCl3的最佳浓度在0.5至1 mM之间变化。本研究中使用的细胞毒性试验简单、易于实现，并能提供有关特定生物和化学产品细胞毒性剂量的可观信息。
