Hassen A, Hachicha R, Jedidi N, Agbalika F, Harteman P
Institut National de Recherche Scientifique et Technique (INRST), Vandoeuvre, Nancy.
Arch Inst Pasteur Tunis. 1991 Jul-Oct;68(3-4):261-8.
A method of detection of enteric viruses in milk was studied. The high protein content of milk and the protein nature of enterovirus allowed the detection of these viruses using the organic acid flocculation method. The poliovirus type 1 (Mahoney strains) and the E.C.H.O.1 isolated from the environment were used as virus model and were inoculated to creamed, half-creamed and whole UHT commercialized milk. The method consists on a milk sample clarification with acid precipitation and centrifugation. The clarified extract is reduced to a final volume of 10 to 15 ml after addition of beef extract powder and protein precipitation. This technique allows the recovery of 26 to 36% of poliovirus type 1 and 10 to 46% of E.C.H.O.1 viruses. In this work, the ferric chloride (FeCl3), added in 0.5 to 1 mM final concentration, was used as an adjuvant for the organic acid precipitation.
研究了一种检测牛奶中肠道病毒的方法。牛奶的高蛋白含量和肠道病毒的蛋白质性质使得可以使用有机酸絮凝法检测这些病毒。将1型脊髓灰质炎病毒（马奥尼株）和从环境中分离出的E.C.H.O.1用作病毒模型，并接种到商业化的高脂、半高脂和全脂超高温灭菌牛奶中。该方法包括用酸沉淀和离心对牛奶样品进行澄清。在加入牛肉浸粉和蛋白质沉淀后，将澄清的提取物浓缩至最终体积为10至15毫升。该技术可回收26%至36%的1型脊髓灰质炎病毒和10%至46%的E.C.H.O.1病毒。在这项工作中，最终浓度为0.5至1 mM的氯化铁（FeCl3）用作有机酸沉淀的佐剂。
