Jayasundar R, Hall L D, Bleehen N M
Medical Research Council Clinical Oncology and Radiotherapeutics Unit, Cambridge, UK.
NMR Biomed. 1992 Nov-Dec;5(6):360-3. doi: 10.1002/nbm.1940050607.
The objective of this study was to compare pH measurements made in biological samples using 31P NMR (pHNMR) with those made with a novel, dye-based fibreoptic pH measurement system (pHF), which is compatible with use in electromagnetic fields without field perturbation. Using protein-free model solutions, pHNMR was calibrated against pHF, giving a correlation coefficient of 0.969 and a mean difference (+/- SD) between pHNMR and pHF of 0.037 +/- 0.054 over the pH range 6.8-7.7. Further calibration of pHNMR with pHF was carried out for human red blood lysates and then pHNMR was compared with pHF for whole, packed red blood cells over the pH range 7.0-7.8. Values for pHNMR, the intracellular pH, were consistently lower than for pHF, the extracellular pH, by a mean (+/- SD) of 0.15 +/- 0.02 units. A close correlation of extracellular pHNMR with pHF was demonstrated for a blood sample exhibiting two P(i) peaks, over the pH range 7.03-7.71. We conclude that concurrent use of NMR and the fibreoptic pH meter provides a reliable method of simultaneous measurement of intracellular and extracellular pH in biological systems.
本研究的目的是比较使用31P核磁共振（pHNMR）对生物样品进行的pH测量结果与使用一种新型的基于染料的光纤pH测量系统（pHF）所测得的结果，该光纤pH测量系统可在不干扰电磁场的情况下用于电磁场环境。使用无蛋白模型溶液，对pHNMR相对于pHF进行校准，在6.8 - 7.7的pH范围内，相关系数为0.969，pHNMR与pHF之间的平均差异（±标准差）为0.037 ± 0.054。对人红细胞裂解液进行了pHNMR与pHF的进一步校准，然后在7.0 - 7.8的pH范围内，将pHNMR与pHF用于全血、浓缩红细胞进行比较。pHNMR（细胞内pH）的值始终比pHF（细胞外pH）低，平均（±标准差）为0.15 ± 0.02个单位。对于一个呈现两个无机磷酸盐（P(i)）峰的血样，在7.03 - 7.71的pH范围内，证明细胞外pHNMR与pHF密切相关。我们得出结论，同时使用核磁共振和光纤pH计提供了一种在生物系统中同时测量细胞内和细胞外pH的可靠方法。
