一种将薄膜包埋于组织树脂中的方法。

A method for embedding thin membranes in historesin.

作者信息

Shimotsuma M, Schoefl G I

机构信息

John Curtin School of Medical Research, Australian National University, Canberra, ACT.

出版信息

Biotech Histochem. 1992 Nov;67(6):377-9. doi: 10.3109/10520299209110054.

DOI:10.3109/10520299209110054

PMID:1489843

Abstract

A method is described for flat-embedding thin membranous tissues in Historesin. It allows easy orientation for sectioning large areas parallel to the surface. Selected fields can be monitored from the unfixed specimen, throughout preparation, to mounting on the microscope slide. For cross-sectioning, the flat-embedded tissue can be stacked and re-embedded to increase the amount of material examined per section.

摘要

本文描述了一种将薄的膜状组织平嵌入Historesin的方法。它便于在平行于表面的大区域进行切片时确定方向。从未固定的标本开始，在整个制备过程直至安装到显微镜载玻片上，都可以对选定的区域进行监测。对于横断面切片，平嵌入的组织可以堆叠并重新嵌入，以增加每片所检查的材料量。