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Determination of beta-carotene in plasma, blood cells and buccal mucosa by electrochemical detection.

作者信息

Murata T, Tamai H, Morinobu T, Manago M, Takenaka A, Takenaka H, Mino M

机构信息

Department of Pediatrics, Osaka Medical College, Japan.

出版信息

Lipids. 1992 Nov;27(11):840-3. doi: 10.1007/BF02535860.

DOI:10.1007/BF02535860
PMID:1491599
Abstract

The beta-carotene concentrations in plasma, blood cells and buccal mucosal cells were determined by high-performance liquid chromatography with electrochemical detection. This method was 1,000 times more sensitive than the conventional spectrophotometric method. Polymorphonuclear cells and red blood cells had lower beta-carotene levels than the other cells. After oral administration of 580 mg/day of all-trans beta-carotene to human male volunteers for a week, the beta-carotene concentrations in all cell types increased at least several times above the original levels.

摘要

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本文引用的文献

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Protein measurement with the Folin phenol reagent.使用福林酚试剂进行蛋白质测定。
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Can dietary beta-carotene materially reduce human cancer rates?膳食中的β-胡萝卜素能显著降低人类患癌率吗?
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beta-Carotene: an unusual type of lipid antioxidant.β-胡萝卜素:一种特殊类型的脂质抗氧化剂。
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Bioavailability of beta-carotene in humans.人体内β-胡萝卜素的生物利用度。
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8
Determination of alpha-tocopherol in buccal mucosal cells using an electrochemical detector.使用电化学检测器测定颊黏膜细胞中的α-生育酚。
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Inhibition of adriamycin-promoted microsomal lipid peroxidation by beta-carotene, alpha-tocopherol and retinol at high and low oxygen partial pressures.β-胡萝卜素、α-生育酚和视黄醇在高氧分压和低氧分压下对阿霉素促进的微粒体脂质过氧化的抑制作用。
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10
Lycopene as the most efficient biological carotenoid singlet oxygen quencher.番茄红素是最有效的生物类胡萝卜素单线态氧猝灭剂。
Arch Biochem Biophys. 1989 Nov 1;274(2):532-8. doi: 10.1016/0003-9861(89)90467-0.