Vile G F, Winterbourn C C
Department of Pathology, Christchurch School of Medicine, Christchurch Hospital, New Zealand.
FEBS Lett. 1988 Oct 10;238(2):353-6. doi: 10.1016/0014-5793(88)80511-8.
Iron-dependent peroxidation of rat liver microsomes, enhanced by adriamycin, was measured in the presence of increasing concentrations of alpha-tocopherol, beta-carotene and retinol at low and high pO2. beta-Carotene and alpha-tocopherol inhibited lipid peroxidation by more than 60% when present at concentrations greater than 50 nmol/mg microsomal protein at both high and low pO2. Retinol inhibited peroxidation by 39% at concentrations greater than 100 nmol/mg microsomal protein. This maximal level of inhibition by retinol was unaltered by pO2. However, beta-carotene was more effective than alpha-tocopherol or retinol at a pO2 of 4 mmHg, whereas alpha-tocopherol was more effective under aerobic conditions. Since adriamycin-dependent lipid peroxidation is maximal at low pO2, beta-carotene may play a role in protecting against this process.
在低氧分压和高氧分压条件下，于大鼠肝微粒体中加入浓度不断增加的α-生育酚、β-胡萝卜素和视黄醇，测定阿霉素增强的铁依赖性过氧化反应。在高氧分压和低氧分压条件下，当β-胡萝卜素和α-生育酚的浓度大于50 nmol/mg微粒体蛋白时，它们对脂质过氧化的抑制作用超过60%。当视黄醇浓度大于100 nmol/mg微粒体蛋白时，其对过氧化反应的抑制率为39%。视黄醇的这种最大抑制水平不受氧分压的影响。然而，在氧分压为4 mmHg时，β-胡萝卜素比α-生育酚或视黄醇更有效，而在有氧条件下α-生育酚更有效。由于阿霉素依赖性脂质过氧化在低氧分压时最大，β-胡萝卜素可能在预防这一过程中发挥作用。
