Lee M G, Chung K Y, Lee J D, Shin J S, Whang K K, Awh O D, Kim S J, Lee J B
Department of Dermatology, Yonsei University College of Medicine, Seoul, Korea.
Acta Derm Venereol. 1993 Aug;73(4):276-9. doi: 10.2340/0001555573276279.
Immunoscintigraphy with radiolabelled monoclonal antibodies has been widely used to detect solid tumours. The purpose of this study was to investigate its potential for the specific localization of syphilitic lesions. F(ab')2 fragments were prepared from murine monoclonal antibodies against Treponema pallidum produced in our laboratory and labelled with 131I. Bilateral testicular infections were created in rabbits by inoculation with T. pallidum or Staphylococcus aureus. Seven to 10 days after inoculation, radiolabelled antibodies were injected intravenously. Serial gamma images were then taken 2 h after the injection and at 24 h intervals thereafter. Beginning as early as 2 h post-injection, the testicles could be visualized with either specific or non-specific antibodies. Gamma images in the monoclonal antibody-treated, T. pallidum-infected group persisted up to 48 h post-injection. Lesions were not discernible from background in the S. aureus-infected group injected with the monoclonal antibody and the S. aureus-infected and T. pallidum-infected groups injected with the polyclonal antibody at 24 h post-injection or later. Therefore, due to its ability to differentiate between specific and non-specific antibody-generated images from 24 h post-injection, immunoscintigraphy using monoclonal antibodies specific for T. pallidum may be employed as one of the methods to diagnose difficult cases of syphilitic internal organ involvement as well as syphilis infection in seronegative HIV-infected patients.
用放射性标记的单克隆抗体进行免疫闪烁显像已广泛用于检测实体瘤。本研究的目的是探讨其在梅毒病变特异性定位方面的潜力。从我们实验室制备的抗梅毒螺旋体鼠单克隆抗体中制备F(ab')2片段，并用131I进行标记。通过接种梅毒螺旋体或金黄色葡萄球菌在兔身上造成双侧睾丸感染。接种后7至10天，静脉注射放射性标记的抗体。然后在注射后2小时及此后每隔24小时拍摄系列γ图像。早在注射后2小时，用特异性或非特异性抗体均可使睾丸显影。单克隆抗体处理的梅毒螺旋体感染组的γ图像在注射后持续长达48小时。在注射单克隆抗体的金黄色葡萄球菌感染组以及注射多克隆抗体的金黄色葡萄球菌感染组和梅毒螺旋体感染组中，在注射后24小时及以后，病变与背景无法区分。因此，由于其能够在注射后24小时区分特异性和非特异性抗体产生的图像，使用针对梅毒螺旋体的单克隆抗体的免疫闪烁显像可作为诊断梅毒内脏器官受累疑难病例以及血清阴性HIV感染患者梅毒感染的方法之一。
