Arruda L K, Chapman M D
Department of Medicine, University of Virginia, Charlottesville.
Exp Appl Acarol. 1992 Nov;16(1-2):129-40. doi: 10.1007/BF01201496.
Exposure to mites other than Dermatophagoides spp., particularly Blomia tropicalis and Euroglyphus maynei, has been increasingly recognized as a cause of asthma. Positive skin tests and serum IgE antibodies to B. tropicalis have been reported in asthmatic patients from several areas of the world, including São Paulo (Brazil), Hong Kong and Tampa (Florida, USA). Analysis of B. tropicalis extracts showed undetectable levels of the major Group I and Group II Dermatophagoides spp. allergens. Immunoabsorption experiments showed that most of the IgE antibodies to B. tropicalis (approximately 70%) reacted with species-specific allergens. Murine monoclonal antibodies to B. tropicalis could present antigens that were recognized by human IgG antibodies. Sensitization to E. maynei has been reported in Europe, North and South America and Australia. Analysis of four different E. maynei extracts by ELISA and RIA showed that E. maynei produces an allergen that is antigenically related to Dermatophagoides Group I allergens. The amino acid sequence of this allergen (Eur m I) has recently been reported. Further identification and purification of B. tropicalis and E. maynei allergens is required to develop specific assays for measuring these allergens in dust samples. This will make it possible to investigate the relationship between exposure to B. tropicalis or E. maynei and the development of sensitization and allergic disease.
接触除嗜皮螨属螨类之外的其他螨类，尤其是热带无爪螨和梅氏真足螨，已日益被认为是哮喘的一个病因。在世界多个地区的哮喘患者中，包括圣保罗（巴西）、香港和坦帕（美国佛罗里达州），均已报告对热带无爪螨的皮肤试验阳性和血清IgE抗体阳性。对热带无爪螨提取物的分析显示，主要的第I组和第II组嗜皮螨属变应原水平检测不到。免疫吸附实验表明，大多数针对热带无爪螨的IgE抗体（约70%）与种特异性变应原发生反应。针对热带无爪螨的鼠单克隆抗体可呈递能被人IgG抗体识别的抗原。在欧洲、南北美洲和澳大利亚均已报告对梅氏真足螨致敏。通过酶联免疫吸附测定（ELISA）和放射免疫分析（RIA）对四种不同的梅氏真足螨提取物进行分析表明，梅氏真足螨产生一种与嗜皮螨属第I组变应原具有抗原相关性的变应原。最近已报告了这种变应原（Eur m I）的氨基酸序列。需要进一步鉴定和纯化热带无爪螨和梅氏真足螨变应原，以开发用于测量灰尘样本中这些变应原的特异性检测方法。这将有可能研究接触热带无爪螨或梅氏真足螨与致敏及过敏性疾病发生之间的关系。
