HEIDELBERGER M, GRAFF S, HAAGENSEN C D
J Exp Med. 1952 Apr;95(4):333-40. doi: 10.1084/jem.95.4.333.
Antisera to the mouse milk factor (F), containing up to 0.5 mg. of antibody nitrogen per ml. are readily obtainable in rabbits, especially when adjuvants are added to the antigen. Quantitative data on the anti-F content of the sera are given, also on the total nitrogen precipitated by the non-casein fraction of C57 mouse milk which does not contain F. The antisera were also absorbed with casein fractions but the difficulties in the way of quantitative analysis were not overcome. Antisera to fractions of C57 mouse milk were also obtained and the reactions of F and the C57 proteins with these sera were studied. The results, as far as they go, are in accord with the earlier belief that the milk factor possesses an immunological specificity not present in normal mouse tissues.
抗小鼠乳因子（F）血清，每毫升含高达0.5毫克抗体氮，在兔体内很容易获得，尤其是在抗原中添加佐剂时。给出了血清中抗F含量的定量数据，以及不含F的C57小鼠乳非酪蛋白部分沉淀的总氮量。这些抗血清也用酪蛋白部分进行了吸收，但定量分析方面的困难并未克服。还获得了针对C57小鼠乳各部分的抗血清，并研究了F和C57蛋白与这些血清的反应。就目前所得结果而言，与早期观点一致，即乳因子具有正常小鼠组织中不存在的免疫特异性。
