Petkov V D, Mosharrof A H, Kehayov R, Petkov V V, Konstantinova E, Getova D
Institute of Physiology, Bulgarian Academy of Sciences, Sofia.
Methods Find Exp Clin Pharmacol. 1992 Oct;14(8):593-605.
The effects of cytidine (5') diphosphocholine (CDP-choline) on learning and memory were studied using conditioned reflex methods for passive avoidance and active avoidance with punishment reinforcement (step-through, step-down, shuttle box and maze), for active avoidance with alimentary reinforcement (staircase maze), and the Morris water maze. The majority of experiments involved comparative studies of the nootropic drugs meclofenoxate and/or piracetam. CDP-choline was administered orally, in some of the experiments also intraperitoneally, at doses of 10-500 mg/kg body weight once or twice daily for 5 or 7 days. In separate cases only single doses were administered. Trainings started one hour after the last dose of the drugs. Retention tests were given 3 h, 24 h, 7 days or 10 days after training. The results obtained with the different methods document CDP-choline's ability to improve learning and memory in rats and mice. No essential differences in the effects of CDP-choline were established upon oral and intraperitoneal administration of the drug. The learning- and memory-facilitating effects of CDP-choline were similar to those of meclofenoxate and piracetam. The results of the present study permit us to define CDP-choline as a substance capable of improving cognitive levels.
采用被动回避和主动回避（带有惩罚强化，如穿梭箱、阶梯式迷宫、跳台、避暗和 Morris 水迷宫）的条件反射方法，以及带有食物强化的主动回避（楼梯迷宫），研究了胞苷（5'）二磷酸胆碱（CDP-胆碱）对学习和记忆的影响。大多数实验涉及对促智药甲氯芬酯和/或吡拉西坦的比较研究。CDP-胆碱通过口服给药，在一些实验中也通过腹腔注射给药，剂量为 10-500mg/kg 体重，每天一次或两次，持续 5 或 7 天。在个别情况下只给予单次剂量。训练在最后一剂药物给药 1 小时后开始。在训练后 3 小时、24 小时、7 天或 10 天进行记忆测试。用不同方法获得的结果证明了 CDP-胆碱改善大鼠和小鼠学习和记忆的能力。口服和腹腔注射该药物后，CDP-胆碱的作用未发现本质差异。CDP-胆碱促进学习和记忆的作用与甲氯芬酯和吡拉西坦相似。本研究结果使我们能够将 CDP-胆碱定义为一种能够提高认知水平的物质。
